脱矿牙本质基质—富血小板血浆凝胶构建牙周骨组织工程支架的初步研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (12): 1184-1187.

脱矿牙本质基质—富血小板血浆凝胶构建牙周骨组织工程支架的初步研究

侯宗鹏¹,郭良微^1*,周晋²,孙金环¹

1. 哈尔滨医科大学附属口腔医院牙周病科黑龙江哈尔滨 150001;
2. 哈尔滨医科大学附属第一医院血液科黑龙江哈尔滨 150001

收稿日期:2015-06-18 出版日期:2015-12-28 发布日期:2016-03-21
通讯作者: 郭良微,E-mail:glw70@hotmail.com
作者简介:侯宗鹏(1988~),男,山东人,硕士,主要从事牙周病的临床研究工作。
基金资助:
黑龙江省自然科学基金项目(编号:D200831)
黑龙江省博士后基金资助(编号:LBH-Z11065)

Preliminary Study of Demineralized Dentin Matrix- Platelet-rich Plasma Gel Applying to Scaffold Material of Periodontal Bone Tissue Engineering.

HOU Zong-peng¹, GUO Liang-wei¹, ZHOU Jin², Sun Jin-huan¹.

1. Department of Periodontology, The Affiliated Stomatological Hospital, Harbin Medical University, Harbin 150001, China;
2. Department of Hematology, The First Hospital of Harbin Medical University, Harbin 150001, China

Received:2015-06-18 Online:2015-12-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:评价自制脱矿牙本质基质(DDM)对富血小板血浆(PRP)作为生长因子缓释系统的影响。方法:二次离心法制备PRP凝胶,分为3组:A:PRP/DDM,B:PRP, C:全血。通过酶联免疫吸附法(ELISA)分别测定2周内单位体积各组凝胶释放的血小板衍化生长因子(PDGF)、转化生长因子-β(TGF-β)的水平。扫描电子显微镜下观察PRP/DDM凝胶的空间结构。将DDM植入SD大鼠股部肌肉陷窝内,1、3周后取材行组织学切片,观察有无毒性反应。结果:PRP/DDM凝胶较其两组持续释放PDGF和TGF-β的时间较长,组间差异有显著的统计学意义(P<0.05);经扫描电镜下观察及组织切片评估PRP与DDM结合紧密、有序;DDM具有很好的生物相容性。结论:DDM与PRP结合能够更好的促进生长因子的缓慢释放。

关键词: 脱矿牙本质基质, 富血小板血浆凝胶, 生长因子, 缓释系统

Abstract: Objective: To evaluate the effects of demineralized dentin matrix (DDM) to the platelet-rich plasma (PRP) gel delivery system for growth factors. Methods: After double centrifugation for preparation of PRP, the concentrations of platelet-derived growth factor (PDGF) and transforming growth factor-β (TGF-β) in the whole blood gel (group C), PRP gel (group B), and PRP/DDM gel (group A) were measured in two weeks by enzyme-linked immunosorbent assay (ELISA). The structure of prepared PRP/DDM gel was observed by scanning electron microscopy (SEM). DDM was implanted into the pit of femoral muscles of SD rats and tissue sections were performed to study the toxicity reaction. Results: Compared with other two groups, PRP/DDM had a much longer time to continuously release the growth factors (P<0.05). Through SEM and tissue section, it was found that PRP and DDM combined with each other closely and orderly and DDM had a fine biocompatibility. Conclusion: The combination of PRP and DDM can better slower the release of growth factors.

中图分类号:

R781.4

侯宗鹏,郭良微,周晋,孙金环. 脱矿牙本质基质—富血小板血浆凝胶构建牙周骨组织工程支架的初步研究[J]. 口腔医学研究, 2015, 31(12): 1184-1187.

HOU Zong-peng, GUO Liang-wei, ZHOU Jin, Sun Jin-huan.. Preliminary Study of Demineralized Dentin Matrix- Platelet-rich Plasma Gel Applying to Scaffold Material of Periodontal Bone Tissue Engineering.[J]. Journal of Oral Science Research, 2015, 31(12): 1184-1187.

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