口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (7): 666-669.

• 基础研究论著 • 上一篇    下一篇

柠檬精油对牙周病主要致病菌粘附作用影响的体外研究

边可胤1,张向宇2*,靳趁心2,李唐萍2   

  1. 1. 天津市口腔医院儿童牙病科 天津 300041;
    2. 天津医科大学口腔医学院
  • 收稿日期:2014-12-29 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 张向宇,E-mail:doctorzxy@sina.cn
  • 作者简介:边可胤(1975~ ),女,天津人,硕士,副主任医师,主要从事儿童口腔医学临床及基础的研究工作。
  • 基金资助:
    天津市卫生局科技基金(编号:2011ky16)

Adhesion Effects of Lemon Essential Oil on Main Pathogens of Periodontal Disease in Vtro

BIAN Ke-yin, Zhang Xiang-yu, Jin Chen-xin, et al   

  1. Tianjin Stomatological Hospital,Tianjin 300041
  • Received:2014-12-29 Online:2015-07-28 Published:2015-07-28

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摘要： 目的:研究柠檬精油(LEO)对牙周病主要致病菌黏附作用的影响,探讨其用于预防和治疗牙周病的可行性。方法:光滑聚苯乙烯表面黏附的影响:将菌液与实验分组中各组药液混合培养于96孔板48h,检测各孔内细菌附着情况;蛋白包被聚苯乙烯表面黏附的影响:将菌液与实验分组中各组药液混合培养于形成蛋白包被表面的96孔板48h,检测各孔内细菌附着情况。结果:光滑聚苯乙烯表面黏附的影响:Aa、Pi中LEO组与对照组比较差异无统计学意义,Pg、Fn中LEO组与对照组比较差异有统计学意义(P<0.05);蛋白包被聚苯乙烯表面黏附的影响:Aa、Pg、Pi、Fn中LEO组与对照组比较差异有统计学意义(P<0.05),LEO能抑制Aa、Pg、Pi、Fn在蛋白包被表面的黏附,对Pg、Fn在光滑聚苯乙烯表面的黏附也有抑制作用。结论:LEO能抑制病原菌对牙面的黏附,阻断牙菌斑的形成,具有预防牙周病的作用。

关键词: 柠檬精油, 牙周病, 致病菌, 黏附

Abstract: Objective: To study the effect of lemon essential oil (LEO) on the adhesion of main pathogenic bacteria of periodontal disease, to explore its feasibility for the prevention and treatment of periodontal disease. Methods: Effect on the smooth polystyrene surface: The bacteria liquid and experimental groups of each component in the liquid mix were cultured in the 96 well plate for 48h. Bacteria adhesion was tested protein coated polystyrene adhesion surface: The bacteria solution and experimental group of each component in the liquid mix were cultured in the protein coated 96 well plates for 48h, Bacteria adhesion was tested. Results: Effect of smooth polystyrene surface adhesion: coneerning to Aa, Pi, there was no statistical significance between the LEO group and the control group; coneerning to Pg, Fn, there was significant difference between the LEO group and the control group (P<0.05); Protein coated polystyrene adhesion surface: coneerning to Aa, Pg, Pi, Fn, there was significant difference between LEO group and the control group (P<0.05). LEO could inhibit the adhesion of Aa, Pg, Pi, Fn on the protein coated surface, can inhibit the adhesion of Pg, Fn on the smooth surface. Conclusion: LEO can block the adhesion of pathogenic bacteria on the teeth surface, block the formation of the dendal plaque , and can prevent periodontal disease.

Key words: LEO, Periodontal disease, Pathogen, Adhesion

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