口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (6): 595-599.DOI: 10.13701/j.cnki.kqyxyj.2019.06.020

• 口腔种植学研究 • 上一篇    下一篇

钛合金微弧氧化载乳铁蛋白复合膜层植入体的动物实验研究

张译丹1,肖月2*,李慕勤1*,姚海涛3,岳申申1,郑玉婧1   

  1. 1. 佳木斯大学黑龙江省高校生物医学材料重点实验室 黑龙江 佳木斯 154007;
    2. 佳木斯大学附属第二医院 黑龙江 佳木斯 154007;
    3. 佳木斯大学基础医学院病理教研室 黑龙江 佳木斯 154007
  • 收稿日期:2018-11-21 出版日期:2019-06-28 发布日期:2019-06-27
  • 通讯作者: 肖月,E-mail:602000365@qq.com
    李慕勤,E-mail:jmsdxlimuqin@163.com
  • 作者简介:张译丹(1992~ ) ,女 ,黑龙江人,硕士,医师,研究方向:口腔临床医学。
  • 基金资助:
    国家自然科学基金(编号:31370979)

Biocompatibility of Lactoferrin Composite Membrane on Pure Titanium Surface Treated by Micro-arc Oxidation Technology

ZHANG Yi-dan1,XIAO Yue2*,LI Mu-qin1*,YAO Hai-tao3,YUE Shen-shen1,ZHENG Yu-jing1   

  1. 1. Key Laboratory of Biomedical Materials, Jiamusi University, Jiamusi 154007, China;
    2. Second Affiliated Hospital of Jiamusi University, Jiamusi 154007, China;
    3. Pathological Teaching and Research Office, School of Basic Medicine, Jiamusi University, Jiamusi 154007, China.
  • Received:2018-11-21 Online:2019-06-28 Published:2019-06-27

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摘要： 目的:探讨钛合金微弧氧化经硅烷耦联剂载人和牛乳铁蛋白,其对骨结合能力的影响。方法:以钛合金微弧氧化硅烷为对照A组,实验组分别是钛合金-MAO-硅烷-牛乳铁蛋白(BLF )B组,钛合金-MAO-硅烷-人乳铁蛋白(HLF)C组,分别植入随机白兔下颌骨内。通过锥形束三维影像设备(CBCT)观察钛植体与骨组织结合情况,通过扫描电镜观察植入后新生骨厚 度和种植体-骨界面的形貌。结果:通过CBCT的观察随着时间的增加,种植体周围骨密度增高,透射影像范围缩小,同期(C)组较(B)组植体周围骨密度增高,透射影像范围缩小,(B)组比(A)组较好。表明其骨组织愈合较好的是8周 的(C)组,(B)组较(A)组好。扫描电镜显示同期(C)组复合膜层均匀分布植体表面,较(A)(B)两组Ca、P含量增多,新生骨量增多,复合膜层元素更广泛地渗透进周围骨组织中。结论:钛合金微弧氧化硅烷耦联载人乳铁蛋白复合膜层,缩短骨结合时间,更具骨诱导能力。

关键词: 钛合金, 微弧氧化, 硅烷, 乳铁蛋白, 骨结合

Abstract: Objective: To investigate the effect of microarc oxidation of titanium alloy with silane coupling agent manned and bovine lactoferrin on bone binding ability. Methods: Titanium alloy microarc oxidized (MAO) silane was used as control group (A). Experiment groups were: titanium alloy-MAO-silane-bovine lactoferrin (BLF) group (B) and titanium alloy-MAO-silane-human lactoferrin (HLF) group (C). They were implanted into the mandible of rabbits at random. Through cone beam three-dimensional imaging equipment (CBCT), the bonding between titanium implant and bone tissue was observed. And through scanning electron microscopy, the thickness of new bone and the morphology of implant-bone interface after implantation were observed. Results: Through CBCT, with the increase of time, the bone density around the implant increased and the range of transmission image narrowed. Group (C) was better than group (B), and group B was better than group A. After 8 weeks, the bone tissue healing was better in group (C), and group (B) was better than group (A). Scanning electron microscopy showed that the composite membranes in group (C) distributed evenly on the implant surface, and compared with group (A) and (B), the contents of Ca and P increased, the new bone quantity increased, and the elements of the composite membranes penetrated into the surrounding bone tissue more extensively. Conclusion: Titanium alloy microarc oxidation silane coupled with human lactoferrin composite membrane could shorten the time of bone binding and might have more osteoinductive ability.

Key words: Ttitanium alloy, Micro-arc oxidation, Silane coupling agent, lactoferrin synostosis