Abstract: Objective: To study the proliferation and differentiation of human placenta-derived mesenchymal stem cells (HPMSCs) combined with Bio-oss powder,and investigate the feasibility of constructing tissue engineered bone by HPMSCs as seed cells and the Bio-oss powder as scaffold material. Methods: Human placenta-derived mesenchymal stem cells were dissociated and cultured under sterile conditions and detected for the ability of differentiation. They were divided into two groups, the group of combination of cells and Bio-oss as scaffold material and the group of cells alone. Cell morphology, proliferation and differentiation in the two groups were observed and compared. Results: There was no significant difference between two groups on the cell morphology, proliferation and differentiation ability. Conclusion: It is feasible to utilize HPMSCs as seed cells and Bio-oss as scaffolds to construct tissue engineered bone.

Key words: Bone tissue engineering, HPMSCs , Bio-oss powder