Effect of Benzalkonium Chloride Modified Adhesive on Long-term Bond Strength of Dentin-resin

doi:10.13701/j.cnki.kqyxyj.2021.04.015

Abstract

Abstract: Objective: To evaluate the effect of benzalkonium chloride (BAC) modified adhesive on the long-term bond strength of dentin-resin. Methods: BAC was added to the 8th generation single bond universal (SBU) of American 3M to make modified adhesives with different concentrations. One hundred third molars stored in thymol solution were randomly divided into 10 groups (n=10): A and A1 groups: 0.25% BAC; B and B1 groups: 0.5% BAC; C and C1 groups: 1% BAC; D and D1: 2% BAC; and E and E1: control group. The specimens in groups A, B, C, D, and E were subjected to shear strength test (SBS) and the fracture mode was counted. The specimens in groups A1, B1, C1, D1, and E1 were subjected to micro-tensile test (μTBS) and the fracture mode was counted. Results: In the shear strength and micro-tensile strength experiments, the strength of the treatment group was significantly lower than that of the control group (P<0.05). The results of group D and group C were statistically different (P=0.01). Group D1 was statistically significant compared with group B1 and group C1 (P<0.05). The fracture mode was mainly mixed fracture, followed by bonding surface fracture and cohesive fracture. Conclusion: In the full-etching bonding mode, adding benzalkonium chloride to the resin adhesive cannot improve the long-term bonding strength between the dentin and the resin, which may be caused by the damaged physical and chemical properties of the adhesive.

Key words: benzalkonium chloride, matrix metalloproteinases, bonding strength, dentin

MOU Wenbo, CHENG Yao, DONG Bo. Effect of Benzalkonium Chloride Modified Adhesive on Long-term Bond Strength of Dentin-resin[J]. Journal of Oral Science Research, 2021, 37(4): 344-348.

References

[1] 贾玲玲,万乾炳.基质金属蛋白酶抑制剂的研究进展[J].华西口腔医学杂志,2017,35(2):208-214.
[2] 焦阳,陈吉华.季铵盐化合物在生物医学材料中的应用进展[J].口腔疾病防治,2019,27(5):280-286.
[3] Parthasarathy R, Misra A, Song L, et al. Structure-property relationships for wet dentin adhesive polymers [J]. Biointerphases, 2018, 13(6):061004.
[4] 郑月梅,鄢国伟,周凯璇,等.碳二亚胺预处理对两种酸蚀-冲洗粘接剂粘接性能的影响[J].口腔医学研究,2020,36(11):1055-1059.
[5] Decha N, Talungchit S, Iawsipo P, et al. Synthesis and characterization of new hydrolytic-resistant dental resin adhesive monomer HMTAF [J]. Des Monomers Polym, 2019, 22(1):106-113.
[6] Mazzoni A, Tjäderhane L, Checchi V, et al. Role of dentin MMPs in caries progression and bond stability [J]. J Dent Res, 2015, 94 (2):241-251.
[7] Frassetto A, Breschi L, Turco G, et al.Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability--A literature review [J]. Dent Mater, 2016, 32 (2):e41-e53.
[8] Mazzoni A, Angeloni V, Comba A, et al. Cross-linking effect on dentin bond strength and MMPs activity [J]. Dent Mater, 2018, 34(2):288-295.
[9] Lukomska-Szymanska M, Konieczka M, Zarzycka B, et al. Antibacterial activity of commercial dentine bonding systems against E. faecalis-flow cytometry study [J]. Materials (Basel), 2017, 10(5):481.
[10] Paludo T, Marcondes ML, Souto AA, et al. Effect of grape seed extract-containing phosphoric acid formulations on bonding to enamel and dentin [J]. Braz Oral Res, 2019, 33:e098.
[11] Jain A, Bahuguna R. Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview [J]. J Oral Biol Craniofac Res, 2015, 5(3):212-218.
[12] 刘雯,刘敏,邓婷,等.原花青素对不同深度牙本质粘接耐久性的影响[J].口腔医学研究,2020,36(4):372-376.
[13] Yu HH, Zhang L, Yu F, et al. Epigallocatechin-3-gallate and epigallocatechin-3-O-(3-O-methyl)-gallate enhance the bonding stability of an etch-and-rinse adhesive to dentin [J]. Materials (Basel), 2017, 10(2):183.
[14] 贾玲玲,万乾炳.基质金属蛋白酶抑制剂的研究进展[J]. 华西口腔医学杂志, 2017, 35(2):208-214.
[15] Pashley DH, Tay FR, Breschi L, et al. State of the art etch-and-rinse adhesives [J]. Dent Mater, 2011, 27(1):1-16.
[16] Comba A, Maravic T, Valente L, et al. Effect of benzalkonium chloride on dentin bond strength and endogenous enzymatic activity [J]. J Dent, 2019, 85:25-32.
[17] Sabatini C, Pashley DH. Aging of adhesive interfaces treated with benzalkonium chloride and benzalkonium methacrylate [J]. Eur J Oral Sci, 2015, 123(2):102-107.
[18] Tezvergil-Mutluay A, Agee KA, Uchiyama T, et al. The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs [J]. J Dent Res, 2011, 90(4):535-540.
[19] Flury S, Lussi A, Peutzfeldt A. Long-term bond strength of two benzalkonium chloride-modified adhesive systems to eroded dentin [J]. Biomed Res Int, 2017, 2017:1207208.