Expression of ADORA3 in Periodontitis and Its Regulatory Role by Transcriptomic Analysis

doi:10.13701/j.cnki.kqyxyj.2023.07.009

Abstract

Abstract: Objective: To investigate the expression pattern of adenosine A3 receptor (ADORA3) in periodontitis and its regulatory role. Methods: Animal models of periodontitis were constructed. Alveolar bone loss and the pathological morphology of periodontal tissues were observed by micro-CT and HE staining in each group of mice. The gingival tissues on the ligature-induced and control sides were subjected to transcriptomic sequencing to analyze the Adora3 expression, and then RT-qPCR was used to observe the expression of ADORA3 in human and mice periodontal tissues and macrophages. The correlation of ADORA3 with downstream target genes was analyzed by transcriptomic sequencing and validated by RT-qPCR. Results: Experimental periodontitis model was successfully established in mice, and the expression of Adora3 was upregulated in periodontal tissues on the ligature-induced side. ADORA3 was expressed highly in periodontal tissues from periodontitis, whereas Adora3 was downregulated in the inflammatory microenvironment. Transcriptomic sequencing results showed that Adora3 level was positively correlated with the downstream target gene arrestin beta-2 (ARRB2). And Adora3 level was down-regulated after Arrb2 knockout. Conclusion: Transcriptomic analysis and in vitro experiments verified that ADORA3 was differentially expressed in periodontal tissues and macrophages, which was positively correlation with ARRB2.

Key words: transcriptomic analysis, periodontitis, adenosine A3 receptor, arrestin beta-2

FENG Meiting, LU Jiawei, LUO Lijun. Expression of ADORA3 in Periodontitis and Its Regulatory Role by Transcriptomic Analysis[J]. Journal of Oral Science Research, 2023, 39(7): 613-618.

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