Study on Lugol's Iodine Enhanced Micro-CT Imaging for Evaluating Margin Status of Oral Squamous Cell Carcinoma

doi:10.13701/j.cnki.kqyxyj.2024.09.008

Abstract

Abstract: Objective: To assess the incisal margin status of oral squamous cell carcinoma (OSCC) in three dimensions using Lugol's iodine enhanced Micro-CT imaging. Methods: A total of 68 human OSCC in vitro samples were collected for this study. All samples were stained with 3% solution of Lugol's iodine for 12 hours and underwent Micro-CT scanning with a layer thickness of 50 μm. Subsequently, the Lugol's iodine stained samples were subjected to routine hematoxylin-eosin (HE) staining sections (4 μm) to create digital pathological sections. The mucosal margin (MM) and deep margin (DM) were measured separately on both the imaging and pathological images. Results: Lugol's iodine enhanced Micro-CT imaging can effectively differentiate tumor boundaries in three-dimensional space, providing more accurate measurements of MM and DM compared to two-dimensional pathological sections, thus enabling identification of positive incisal margins and critical incisal margins. Conclusion: Lugol's iodine enhanced Micro-CT imaging allows comprehensive evaluation of OSCC incisal margins in three dimensions, offering more comprehensive and clear information regarding tumor margins which can guide clinical treatment plan selection and prognosis assessment.

Key words: oral squamous cell carcinoma, Micro-CT, Lugol's iodine, 3D

YU Jiaxin, LIU Kunyu, ZHANG Qian, PU Yumei, HU Qingang, XIA Chengwan, WANG Yuxin. Study on Lugol's Iodine Enhanced Micro-CT Imaging for Evaluating Margin Status of Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2024, 40(9): 797-802.

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