3D Bioprinting of HGFs and HUVECs Co-culture System Promotes Vascularization of HUVECs.

doi:10.13701/j.cnki.kqyxyj.2024.04.004

Abstract

Abstract: Objective: To construct a co-culture system of human gingival fibroblasts (HGFs) and human umbilical vein endothelial cells (HUVECs) to promote vascularization of HUVECs. Methods: HGFs and HUVECs were cultured to 3 to 5 passages for experiments. The HUVECs was labeled with a red fluorescent protein by lentiviral transfection. A two-dimensional co-culture system of HGFs and HUVECs was constructed. HGFs and HUVECs were co-cultured at different ratios (4∶1, 1∶1, and 1∶4), and the formation of vascular network was observed under fluorescence microscope. 5% methacrylylated gelatin (GelMA) 30 hydrogel was prepared to construct a three-dimensional co-culture system of HGFs & HUVECs, and the formation of vascular network was observed under laser confocal microscope and quantitative analyzed. HUVECs were separated from three-dimensional co-culture system to assess the proliferation, migration, tube formation, and expression levels of relevant angiogenesis genes after single-culture and co-culture for a certain period. Results: In two-dimensional co-culture, vascular network formation was best when HGFs and HUVECs were cocultured in a ratio of 1∶1. In three-dimensional co-culture, HGFs can promote HUVECs angiogenesis. The co-culture of HGFs & HUVECs had better effects on proliferation, migration, and tube formation compared to the three-dimensional culture of HUVECs alone, and the expression levels of relevant angiogenesis genes were significantly higher. Conclusion: Co-culture of HGFs and HUVECs can guide and promote HUVECs migration and sprouting.

Key words: 3D bioprinting, vascularization, tissue engineering, human umbilical vein endothelial cells, human gingival fibroblasts

LI Junjun, WANG Wen, GUO Huiying, YUAN Changyong, XIA Tingxu, WANG Penglai. 3D Bioprinting of HGFs and HUVECs Co-culture System Promotes Vascularization of HUVECs.[J]. Journal of Oral Science Research, 2024, 40(4): 297-303.

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