Effects of Different Osteogenic Induction Systems on Osteogenic Differentiation of MC3T3-E1 Cells

doi:10.13701/j.cnki.kqyxyj.2024.04.005

Abstract

Abstract: Objective: To determine the optimal osteogenic induction system in vitro for MC3T3-E1 osteoblasts. Methods: Different osteogenic induction systems were constructed, which were dexamethasone 0.1 μmol/L, ascorbic acid 50 μg/mL, and β-sodium glycerophosphate 10 mmol/L in group A; dexamethasone 0.01 μmol/L, ascorbic acid 50 μg/mL, and β-sodium glycerophosphate 10 mmol/L in group B; dexamethasone 1 μmol/L, ascorbic acid 50 μg/mL, and β-sodium glycerophosphate 10 mmol/L in group C; ascorbic acid 50 μg/mL and β-sodium glycerophosphate 10 mmol/L in Group D; and ascorbic acid 100 μg/mL and β-sodium glycerophosphate 5 mmol/L in Group E. MC3T3-E1 cells were induced in vitro for 7 days and 14 days. The effects of alkaline phosphatase staining and alizarin red staining on osteogenic differentiation of MC3T3-E1 were analyzed. The expression levels of osteogenic differentiation related genes (ALP, Runx-2, COL-1, OCN, OPN) were detected by RT-qPCR. Results: There was a significant difference in the optimal quantification of alizarin among groups B, D, and E (P<0.05). The PCR results at 7 days after induction showed that the gene expression levels of ALP, Runx-2, and COL-1 in group C were the highest (P<0.05). PCR results at 14 days after induction showed that the expression of OCN in Group D was the highest (P<0.05). Conclusion: The concentrations of dexamethasone and ascorbic acid have an effect on the osteogenic differentiation of MC3T3-E1 cells. Group C was better for early osteogenesis and Group D was better for late osteogenesis. However, in comprehensive consideration, Group B is recommended for late osteogenesis.

Key words: MC3T3-E1, osteogenic differentiation, inducement system

GUAN Xin, CHEN Anqi, LAI Yingzhen, WU Yongmin, WENG Xinze, WANG Weixin, LEI Yixuan. Effects of Different Osteogenic Induction Systems on Osteogenic Differentiation of MC3T3-E1 Cells[J]. Journal of Oral Science Research, 2024, 40(4): 304-309.

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