SMURF2/β-catenin Axis Promotes Osteogenic Differentiation of Human Periodontal Ligament Cells under Cyclic Stretch

doi:10.13701/j.cnki.kqyxyj.2023.07.010

Abstract

Abstract: Objective: To investigate the effects and mechanisms of Smad ubiquitylation regulatory factor 2(SMURF2) in cyclic stretch-induced osteogenic differentiation of human periodontal ligament cells (hPDLCs). Methods: Cyclic stretch was applied on hPDLCs. The expression of SMURF2 and osteogenic genes were analyzed by qRT-PCR. The expression of SMURF2 and molecules mediating canonical WNT signaling were examined by western blotting. The osteogenic differentiation of hPDLCs was assessed by ALP staining and expression of osteogenic genes. A small interfering RNA was used to knockdown SMURF2 in hPDLCs to investigate effects of SMURF2 on the osteogenic differentiation of hPDLCs and canonical WNT signaling. Results: Expressions of SMURF2, ALP, OPN, OSX, COL-1, and β-catenin were up-regulated in hPDLCs applied cyclic stretch. Activity of ALP was also up-regulated in stretched cells. Depletion of SMURF2 impaired the activity of ALP, down-regulated the expression of ALP,OPN, and OSX genes, and β-catenin protein. However, the protein level of GSK-3β was not changed after SMURF2 depletion. Conclusion: SMURF2 may promote cyclic stretch-induced osteogenic differentiation of hPDLCs through canonical WNT signaling pathway.

Key words: cyclic stretch, human periodontal ligament cells, osteogenic differentiation, smad ubiquitylation regulatory factor 2

ZHANG Xiaocen, CHANG Maolin, ZOU Hao, LIU Xiaoyu, HAN Guangli. SMURF2/β-catenin Axis Promotes Osteogenic Differentiation of Human Periodontal Ligament Cells under Cyclic Stretch[J]. Journal of Oral Science Research, 2023, 39(7): 619-624.

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