Expression and Prognostic Significance of COL7A1 and LAMC2 in Oral Squamous Cell Carcinoma.

doi:10.13701/j.cnki.kqyxyj.2024.07.012

Abstract

Abstract: Objective: To investigate the expression of type Ⅶ Collagen α-1 chain (COL7A1) and laminin γ-2 (LAMC2) in oral squamous cell carcinoma, and to clarify the correlation between the expression of COL7A1 and LAMC2 and clinical parameters and prognosis in OSCC patients. Methods: Immunohistochemistry was used to detect the expression of COL7A1 and LAMC2 in 108 cases of OSCC tissues and 55 cases of adjacent tissues, and to analyze the correlation between COL7A1 and LAMC2 and the clinicopathological features and prognosis of OSCC patients. The expression levels of COL7A1 and LAMC2 in OSCC cell lines were detected by real-time fluorescence quantitative PCR and western blotting. Finally, the bioinformatics database was used for verification. Results: The expressions of COL7A1 and LAMC2 in OSCC tissues were significantly higher than those in adjacent tissues (P<0.001), and the expressions of COL7A1 and LAMC2 were positively correlated (r=0.655,P<0.001). The expression of COL7A1 was related to T stage, TNM stage, and lymph node metastasis (P<0.05), and that of LAMC2 was related to TNM stage and lymph node metastasis (P<0.05). Compared with normal oral keratinocytes, COL7A1 and LAMC2 were overexpressed in OSCC cell lines (P<0.05). Kaplan-Meier analysis showed that the overall survival time of the high expression group of COL7A1 and LAMC2 was shorter than that of the low expression group (P<0.05). Multiple Cox regression analysis showed that COL7A1 and LAMC2 was an independent risk factor for OSCC patients (P<0.05). Conclusion: The increased expression of COL7A1 and LAMC2 in OSCC has certain reference value for the prognosis evaluation of patients with oral squamous cell carcinoma, and is related to the TNM stage and metastasis of the tumor.

Key words: oral squamous cell carcinoma, type Ⅶ collagen, laminin γ2, prognosis

LIU Jiaqi, LIANG Denghuan, ZHANG Jiajia, GUO Chaojie, LIU Yuci, XU Jiang, ZENG Yan, ZHENG Jun. Expression and Prognostic Significance of COL7A1 and LAMC2 in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2024, 40(7): 634-639.

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