Antimicrobial Studies of Liposome Nanoparticles Loaded with Indocyanine Green Against Streptococcus Gordonii In Vitro

doi:10.13701/j.cnki.kqyxyj.2025.02.009

Abstract

Abstract: Objective: To examine the antibacterial activities of liposomeloaded with indocyanine green (ICG) against streptococcus gordonii using photothermal photodynamic processes in vitro. Methods: The nanoprecipitation method was used to encapsulate ICG in liposomes to obtain indocyanine green nanoparticles (ICG NPs).The particle size and polydispersity index were characterized, and the encapsulation rate of ICG NPs was measured. The photothermal characteristics and generation of reactive oxygen species(ROS) of free ICG and ICG nanoparticles were studied in vitro. Crystal violet staining and colony counting were used to assess the antibacterial efficacy of free ICG and ICG nanoparticles against streptococcus gordonii and to evaluate their effect on biofilm. A test for hemolysis was used to determine the biocompatibility of ICG nanoparticles. Results: ICG NPs had an encapsulation rate of 93.7%, an average particle size of (146±2) nm, and a polydispersity coefficient of 0.263. Compared to the control group, the number of colonies and biofilm biomass gradually decreased with the increase of ICG concentration under NIR irradiation (P<0.05). Additionally, the ICG NPs exhibited higher antimicrobial (99%) and anti-biofilm (87%) efficiencies. The amount of intracellular ROS gradually increased after different ICG and ICG NPs concentrations were irradiated by 1 W/cm² of near-infrared light. ICG NPs had a hemolysis rate of only 3.34%. Conclusion: Periodontal streptococcus gordonii and biofilm can be efficiently destroyed by applying ICG NPs for antimicrobial photodynamic and photothermal therapy.

Key words: liposome nanoparticles, ICG, photodynamic therapy, photothermal therapy, streptococcus gordonii

YANG Qing, CAO Shisheng, GUO Surong, MU Xingtong, ZHAO Cailing, ZHANG Juan. Antimicrobial Studies of Liposome Nanoparticles Loaded with Indocyanine Green Against Streptococcus Gordonii In Vitro[J]. Journal of Oral Science Research, 2025, 41(2): 134-139.

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