Effects of Chondroitin Sulfate Proteoglycan on MC3T3-E1 Cell Line Mineralization.

Abstract

Abstract: Objective: To observe the effect of chondroitin sulfate proteoglycan (CSPG) on the alkaline phosphatase (ALP) activity and calcium deposition quantity in pre-osteoblast cell line MC3T3-E1 mineralization process. Methods: MC3T3-E1 cells were cultured with different concentrations of CSPG(0,5,10,15 μg/ml)in induction medium,without CSPG as control group,the other three groups as the experimental group. Cells were cultured for 14 days by ALP staining and activity assay,21 days by alizarin red staining and calcium deposition assay. Results: Compared to the control group,ALP staining and alizarin red staining showed a decreasing trend with the increasing of CSPG concentration in the experimental group;The groups was statistically significant difference between the amount of calcium deposition (P<0.05), no significant difference in ALP activity. Conclusion: In the experimental period,CSPG inhibited calcium deposition of MC3T3-E1 cells in a dose dependent manner.

Key words: Chondroitin sulfate proteoglycan, Alkaline phosphatase, Mineralization , MC3T3-E1

CLC Number:

R781.4

ZHANG Yu, MA Su, LIU Pei-hong, et al. Effects of Chondroitin Sulfate Proteoglycan on MC3T3-E1 Cell Line Mineralization.[J]. Journal of Oral Science Research, 2015, 31(4): 317-319.

References

[1] Francois Lamoureux, Marc Baud'huin, Laurence Duplomb. Proteoglycans:key partners in bone cell biology [J]. BioEssays,2007,29∶758-771
[2] 贾弘禔.生物化学与分子生物学[M].第2版.北京:人民卫生出版社,2010∶104
[3] 高洋,陈海敏,严小军.糖胺聚糖和蛋白相互作用方法学研究进展[J].药物分析杂志,2009,29(12)∶2171-2178
[4] Mauricio Cortes, Alexis T, Baria, et al. Schwartz.Sulfation of chondroitin sulfate proteoglycans is necessary for proper Indian hedgehog signaling in the developing growth plate [J]. Development,2009,136∶1647-1706
[5] Chatles W. Prince, Firoz Rahemtulla, William T. Butler. Metabolism of rat bone proteoglycans in vivo [J]. Biochemical,1983,216∶589-596
[6] Fukae M, Tanabe T, Yamada M. Action of metalloproteinases on porcine dentin mineralization [J]. Calcif Tissue Int. 1994,55∶426-435
[7] 徐春燕,邓辉,叶洁,等.脂多糖刺激的单核巨噬细胞培养上清液对小鼠MC3T3-E1细胞c-fos表达的影响[J].口腔医学研究杂志,2010,26(6)∶786-789
[8] Masayoshi Kamon, Ran Zhao, Kazuichi Sakamoto.Green tea polyphenol (-)-epigallocatechin gallate suppressed he differentiation of murine osteoblastic MC3T3-E1 cells [J]. Cell Biol Int,2010,34(1)∶109-116
[9] Karsdal MA, Neutzsky-Wulff AV, Dziegiel MH, et al.Osteoclasts secrete non-bone derived signals that induce bone formation [J]. Biochem Biophys Res Commun,2008,366(2)∶483-488
[10] Kodama H, Amagai Y, Sudo H, et al. Establishment of a clonal osteogenic cell line from newborn mouse calvaria [J]. Oral Biol,1981,23∶899-901
[11] Nakatoni Y, Tsunoi M , Hakeda Y, et al. Effects of parothyroid hormone on cAM P production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells [J]. Biochem Biophys Res Commun,1984,123(3)∶894-898
[12] Hata R, HoriH, Nagai Y, et al. Selective inhibition of type I collagen synthesis in osteoblastic cells by epidermal growth factor [J]. Endocrinology, 1984, 115(3)∶867-876
[13] Suda H, Kodama H, Yamagai Y,et al. In vitro differentiation and calcification in a new clone osteogenic cell line derived from newborn mouse calvaria [J]. Cell Biol,1983,96(1)∶191-198
[14] 栾静,崔亚洲,周小艳,等.基质小泡蛋白与骨矿化[J].国际骨科学杂志,2011,32(5)∶277-279
[15] Torii Y, Hitomi K, Yamagishi Y,et al.Demonstration of alkaline phosphatase participation in the mineralization of osteoblasts by antisense RNA approach [J]. Cell Biol Int,1996,20(7)∶459-464
[16] Xu W, Xu L, Chen M, et al. The effects of low dose X-irradiation on osteoblastic MC3T3-E1 cells in vitro [J]. BMC Musculoskelet Disord,2012,13∶94
[17] Saori Okahata, Ryuji Yamamoto, Yasuo Yamakoshi. A large chondroitin sulfate proteoglycan,versican,in porcine predentin [J]. Oral Biosciences,2011,53(1)∶72-81
[18] Cheng H,Caterson B,Yamauchi M. Idenfication and immunolocalization of chondroitin sulfate proteoglycans in tooth cementum [J]. Connect Tissue Res,1999,40(1)∶37-47
[19] Yasuhiro Takeuchi, Ttoshio MatsumotoA, Etsuro Ogata. Isolation and characterization of proteoglycans synthesized by mouseoste oblastic cells in culture during the mineralization process [J]. Biochem,1990,266∶15-24