EGFR Is Involved in Lysophosphatidic Acid Induced Integrin β6 Expression in Oral Squamous Carcinoma Cells

Abstract

Abstract: Objective: To investigate the role of EGFR in the process of integrin β6 (ITGB6) expression in oral squamous carcinoma (SAS) cells induced by lysophosphatidic acid (LPA). Methods: SAS cells were cultured in 6-well culture plate and stimulated with 10 μmol/L LPA. The cells were collected for RNA and protein extraction. The mRNA and protein level of ITGB6 were detected by real-time RT-PCR and Western blotting, respectively. The EGFR-specific inhibitor AG1487 was applied to investigate the role of EGFR activation in LPA induced ITGB6 expression.Results: The mRNA level of ITGB6 in SAS cells was statistically upregulated after treated with LPA for 2h, while the protein expression of ITGB6 was obviously increased after incubation with LPA for 4h. The increased phosphorylation of the EGFR was induced by LPA stimulation in SAS cells. EGFR inhibitor AG1478 could block LPA induced ITGB6 mRNA and protein expression in SAS cells. Conclusion: LPA can induce the ITGB6 expression in the oral SAS cells, and EGFR transactivation may be involved in this process.

CLC Number:

R739.8

YIN Hao, DENG Xiao-ling, LI Guo-yang, et al. EGFR Is Involved in Lysophosphatidic Acid Induced Integrin β6 Expression in Oral Squamous Carcinoma Cells[J]. Journal of Oral Science Research, 2015, 31(8): 767-.

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