Effects of PEN-loading Oligodeoxynucleotide on the Cell Cycle and Osteogenic Differentiation of Osteoblastic Cells.

doi:10.13701/j.cnki.kqyxyj.2016.03.022

Abstract

Abstract: Objective: To adopt a new vector PEN-loading ODN and evaluate its effects on the cell cycle and osteogenic differentiation of osteoblastic cells. Methods: Different ratios of MT01/PEN complex were transfected into MG63 cells, while MT01 and S-MT01 were negative controls. After that, the cell cycle, ALP activity, and the expression levels of BMP2 and OCN were detected. Results: In MT01/PEN group, cell percentages in Gl and G2 phase were decreased, while that in S phase was increased (P<0.01). Meanwhile, ALP activity was increased (P<0.01), and the expression of OCN and BMP2 were significantly up-regulated (P<0.05). Conclusion: The application of PEN as a carrier of ODN could promote the osteogenic differentiation of MG63 cells.

Key words: MG63 , ODN , MT01 , Derivative of , PEI, Differentiation

CLC Number:

R781.4

LOU Yi-xin, ZHENG Yi, SHEN Yu-qin, CUI Ye, SUN Han, SUN Xin-hua. Effects of PEN-loading Oligodeoxynucleotide on the Cell Cycle and Osteogenic Differentiation of Osteoblastic Cells.[J]. Journal of Oral Science Research, 2016, 32(3): 301-304.

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