Abstract: Objective: To observe the immunogenic and immunoreactivity of transgenic tomatoes vaccine containing PAcA/CTB against caries by animal experiment. Methods: Eighteen androgenesis BALB/c mice of six to eigh-week-old were randomly divided in three groups and fed transgenic tomatoes fruit tissue (experiment group), control tomatoes fruit tissue (negative control group) or deactivated S.mutans (positive control group). Animals were bled and saliva samples were collected one day before the first immunity and at the 1, 2, 3, 4th week after immunity. Antibody titer was analyzed by ELISA to observe the immunogenic antibody. Results: The PAcA specific IgG antibodies in sera hoisted in the positive control group and the experiment group one week after the first immunity.The antibody titer was significantly higher than those fed with control transgenic tomatoes (P<0.05 or P<0.01) at the 4th week. The antibody titer was significantly different between positive control group and the experiment group at the 2nd, 3rd and 4th weeks after the first immunity. Conclusion: The foreign protein which induced by transgenic tomatoes was immunogenic and could induce immunity responses in BALB/c mice.

Key words: Transgenic tomatoes , Edible vaccine against caries, BALB/c mice