Bioinformatics Analysis of Alternative Splicing Events during Mouse Cementoblast Mineralization

doi:10.13701/j.cnki.kqyxyj.2021.07.012

Abstract

Abstract: Objective: To bioinformational analyze the alternative splicing events and biofunctions and significant signaling pathways of differential alternative splicing genes during mouse cementoblasts (OCCM30) mineralization. Methods: OCCM30 were cultured in mineralized medium for 0, 7, and 14 days. The relative gene expression levels of bone gamma-carboxyglutamate protein (Bglap) and Osterix (Osx) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed to enrich potential biofunctions and significant signaling pathways. Results: Compared with those on day 0, 1702 and 1682 alternative splicing events were detected on day 7 and 14, respectively. Differential alternative splicing genes were associated with protein serine/threonine kinase activity, peptidyl-lysine modification, Wnt signaling pathway, and VEGF (vascular endothelial growth factor) signaling pathway and so on. Conclusion: Cementoblasts were successfully induced by mineralized medium. Using bioinformatics analysis, alternative splicing events were identified and differential alternative splicing genes may exert functions on cementoblast mineralization, which may provide a novel insight for cementogenesis and periodontal regeneration in the future.

Key words: cementoblast, alternative splicing, mineralization

HUANG Hantao, WANG Xiaoxuan, JIANG Chenxi, MA Li, YAO Siqi, LIU Huan, CAO Zhengguo. Bioinformatics Analysis of Alternative Splicing Events during Mouse Cementoblast Mineralization[J]. Journal of Oral Science Research, 2021, 37(7): 632-636.

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