Proteomic Study on Salivary Differences between OSAHS Children and Non-OSAHS Children

doi:10.13701/j.cnki.kqyxyj.2025.04.007

Abstract

Abstract: Objective: To analyze the related information of salivary differential proteins between OSAHS children and non-OSAHS children through proteomics detection technology. Methods: Saliva samples were collected from 6 children with OSAHS [5 males and 1 female, with an average age of (12.00±2.71) years] and 6 non-OSAHS healthy children [3 males and 3 females, with an average age of (10.22±2.05) years]. Total proteins were extracted, data-independent acquisition (DIA) mass spectrometry analysis and protein identification of differential proteins were performed, and finally bioinformatics analysis was conducted on the screened differential proteins. Results: 5636 proteins were identified in the non-OSAHS group and 5608 proteins in the OSAHS group, among which 5582 proteins were present in all groups. A total of 303 up-regulated proteins and 185 down-regulated proteins were screened out as significant differences between the two groups, among which several proteins with significant differences, such as KRT80, CXCL10, KRT19, and SAA2, were selected. Conclusion: There are differences in salivary proteins between OSAHS children and non-OSAHS children, and OSAHS molecular markers may be closely related to its occurrence, development, and pathogenesis.

Key words: obstructive sleep apnea hypopnea syndrome, salivary protein, proteomics, bioinformaticsanalysis , data-independent acquisition technology, differential protein, polysomnography, mouth breathing

Zulihumaer·NUERAIHEMAITI, Maikeliya·PALIHATI, Gulibaha·MAIMAITILI. Proteomic Study on Salivary Differences between OSAHS Children and Non-OSAHS Children[J]. Journal of Oral Science Research, 2025, 41(4): 306-313.

References

[1] 冯梦绮,陈雪慧,葛兮源,等.无鼾男性唾液蛋白昼夜差异的研究[J].中华口腔正畸学杂志,2020,27(3):162-166.
[2] Salzano G, Maglitto F, Bisogno A, et al. Obstructive sleep apnoea/hypopnoea syndrome: relationship with obesity and management in obese patients [J]. Acta Otorhinolaryngol Ital, 2021, 41(2):120-130.
[3] 田润琦,段鹏,苏峰,等.阻塞性睡眠呼吸暂停低通气综合征与卵圆孔未闭相关性的meta分析[J].现代医药卫生, 2024,40(12):2068-2073.
[4] Senaratna CV, Perret JL, Lodge CJ, et al. Prevalence of obstructive sleep apnea in the general population:A systematic review [J].Sleep Med Rev, 2017, 34:70-81.
[5] 林文婷,曾敏,陈积雄,等.老年阻塞性睡眠呼吸暂停低通气综合征患者冠心病发病风险的调查[J].中华老年心脑血管病杂志,2020,22(2):153-155.
[6] Chan KC, Au CT, Hui LL, et al. Childhood OSA is an independent determinant of blood pressure in adulthood: longitudinal follow-up study [J]. Thorax, 2020, 75(5):422-431.
[7] Ingram DG, Singh AV, Ehsan Z, et al. Obstructive sleep apnea and pulmonary hypertension in children [J]. Paediatr Respir Rev, 2017, 23:33-39.
[8] 石劲松,刘芳,陈黔妹.多导睡眠图监测仪评价急性脑梗死患者OSAHS发生率及影响因素的Logistic回归方程分析[J].河北医学,2021,27(11):1877-1881.
[9] 中国儿童OSA诊断与治疗指南制订工作组.中国儿童阻塞性睡眠呼吸暂停诊断与治疗指南(2020)[J].中华耳鼻咽喉头颈外科杂志,2020,55(8):729-747.
[10] 卢燕波,蔡小红,吴军华,等.儿童阻塞性睡眠呼吸暂停低通气综合征相关生物标志物研究进展[J].浙江医学,2022,44(8):882-886.
[11] 张念华.端粒酶调节参与甲状腺癌的分子机制研究[D].山东第一医科大学,2019.
[12] 赵相轩,温锋,卢再鸣.端粒酶调控放射敏感性在恶性肿瘤治疗中的作用进展[J].肿瘤,2018,38(5):498-501.
[13] 由振华,黄锦宏,赵云根,等.端粒酶、γ-干扰素、腺苷酸脱氨酶及结核感染T细胞斑点试验在诊断结核性和恶性胸腔积液中的作用分析[J].海军医学杂志,2024,45(1):53-57.
[14] 周娟.人类PTPN4、ABCF3和PINX1基因的生物学功能研究[D].复旦大学,2011.
[15] 刘焯霖,梁秀龄,张成.神经遗传病学[M].人民卫生出版社,2011.
[16] Finamore A, Peluso I, Cauli O, et al. Salivary stress/immunological markers in Crohn's disease and ulcerative colitis [J]. Int J Mol Sci, 2020, 21(22):8562.
[17] Zian Z, Bakkach J, Barakat A, et al. Salivary biomarkers in systemic sclerosis disease [J]. Biomed Res Int, 2018:3921247.
[18] Tvarijonaviciute A, Zamora C, Ceron JJ, et al. Salivary biomarkers in Alzheimer's disease [J]. Clin Oral Investig, 2020, 24(10):3437-3444.
[19] 朱方清.KRT80在胃癌中的表达及临床意义研究[D].大连医科大学,2017.
[20] Imani MM, Sadeghi M, Khazaie H, et al. Associations between morning salivary and blood cortisol concentrations in individuals with obstructive sleep apnea syndrome:A meta-analysis [J]. Front Endocrinol (Lausanne), 2021, 11:568823.
[21] Tóthová L',Celec P, Mucska I, et al. Short-term effects of continuous positive airway pressure on oxidative stress in severe sleep apnea [J]. Sleep Breath, 2019, 23(3):857-863.
[22] Yan YR, Zhang L, Lin YN, et al. The association of salivary biomarkers with the severity of obstructive sleep apnea and concomitant hypertension [J]. Am J Med Sci, 2019, 357(6):468-473.
[23] 王磊,李保卫,王刚,等.阻塞性睡眠呼吸暂停低通气综合征患者夜间碱反流初步研究[J].山东大学耳鼻喉眼学报,2023,37(6):75-79.
[24] 孙红村,江文博,邱小雯,等.阻塞性睡眠呼吸暂停低通气综合征患者唾液中胃蛋白酶含量测定的研究[J].中国耳鼻咽喉头颈外科,2020,27(1):37-39.
[25] Jeong JH, Guilleminault C, Park CS, et al. Changes in salivary cortisol levels in pediatric patients with obstructive sleep apnea syndrome after adenotonsillectomy [J]. Sleep Med, 2014, 15(6):672-678.
[26] 刘琳,马倩,万光宇,等.CXCL10基因对SH-SY5Y细胞氧化损伤,钙稳态及Nrf2/BNIP3的影响[J].解剖学研究, 2023(6):511-517.
[27] Chen X, Cao W, Zhuang Y, et al. Integrative analysis of potential biomarkers and immune cell infiltration in Parkinson's disease [J]. Brain Res Bull, 2021, 177(12):53-63.
[28] 张新燕,刘英香,赵国栋,等.结直肠癌中SAA2表达与肿瘤免疫微环境的关系[J].中国免疫学杂志,2022,38(20):2495-2500.
[29] 李孟如,王小琴.儿童/青少年口呼吸与口腔微生态的研究进展[J].口腔医学研究,2024,40(6):479-483.
[30] Zhang X, Li X, Xu H, et al. Changes in the oral and nasal microbiota in pediatric obstructive sleep apnea [J]. J Oral Microbiol, 2023, 15(1):2182571.