Role of PI3K/AKT/mTOR/p70S6K Pathway in Drug Resistance of Tongue Squamous Cells

doi:10.13701/j.cnki.kqyxyj.2017.09.007

Abstract

Abstract: Objective: To investigate the role of PI3K/AKT/mTOR/p70S6K signaling pathway in drug resistance of tongue squamous cells. Methods: Tongue squamous cell carcinoma line Cal27 and tongue squamous cell carcinoma cell line Cal27/CDDP with cisplatin resistance were repeatedly treated with the specific inhibitor, LY294002 (PI3K/AKT inhibitor) , Rapamycin (mTOR inhibitor), and LY2584702 (p70S6K inhibitor). Related proteins in PI3K/AKT/mTOR/p70S6K pathway were detected by Western blot. The level of autophagy was detected with Cyto-ID fluorescence staining. Cellular apoptosis was detected by flow cytometry. Results: After applying the pathway inhibitors, the expression level of Beclin1 of the tongue squamous cells were enhanced compared with the control group. Moreover, the ratio of the LC3II to LC3I and the ratio of the Bax to Bcl-2 were increased. The expression of p-AKT, p-mTOR, and p-p70S6K were decreased (P<0.05). The apoptosis and autophagy level were increased in tongue squamous cells, compared with control group. Conclusion: Inhibiting the PI3K/AKT/mTOR/p70S6K signaling pathway shows a significant effect on apoptosis and autophagy in Cal27 and Cal27/CDDP.

Key words: Tongue squamous cells, PI3K/AKT/mTOR/p70S6K, Cisplatin resistance, Autophagy, Apoptosis

CLC Number:

R739.8

ZHANG Si-ying, PAN Shu-ting, SHUAI Fang-yuan, QIU Jia-xuan. Role of PI3K/AKT/mTOR/p70S6K Pathway in Drug Resistance of Tongue Squamous Cells[J]. Journal of Oral Science Research, 2017, 33(9): 938-942.

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