Preparation and Cell Biology Imaging with Tryptophan Carbon Dots

doi:10.13701/j.cnki.kqyxyj.2017.11.020

Abstract

Abstract: Objective: To investigate the imaging characteristics of different cells that labeled by carbon dots (CDs) with multicolor fluorescence properties. Methods: Tryptophan CDs were synthesized by hydrothermal method and characterized by TEM, FT-IR, and fluorescence spectrometer. MTT method was used to detect CDs at different concentrations co-cultured with RAW264.7, MC3T3-E1, and L929 for 24h, which were observed under confocal microscope. The optimum imaging concentration of CDs was determined. Results: TEM showed that CDs were spherical particles with excellent dispersion and particle size of about 3.72nm. FTIR showed that molecular structure of CDs was consisted of carbon, oxygen, hydrogen, and nitrogen elements. Fluorescence spectra showed that at different excitation wavelengths, CDs could present different colors with excitation dependencies. MTT results showed that the cell activity of RAW264.7, MC3T3-E1, and L929 were up to 67%, 79% and 89% when the concentration of CDs was 400mg/L. The results of cell imaging showed that green and red fluorescence images were observed at 488 nm and 543 nm excitation wavelengths. The fluorescence of CDs was mainly detected in cell membrane and cytoplasm of RAW264.7 and L929. The entire MC3T3-E1 cell showed fluorescence, which indicated that the CDs might partially enter into its nucleus. Conclusion: Tryptophan CDs can be applied to cell imaging to facilitate observation and analysis of cell morphology and physiological activities.

Key words: Carbon dots, Cell, Biological imaging

CLC Number:

R780

Tang Qiu-Ling, ZHAO Xiao-huan, YANG Ming-xi, PAN Jia-hui, LI Ge-ge, MENG Yang, WANG Liu-ran, YU Wei-xian. Preparation and Cell Biology Imaging with Tryptophan Carbon Dots[J]. Journal of Oral Science Research, 2017, 33(11): 1213-1217.

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