Experimental Study on Thapsigargin Induced Apoptosis in Tongue Cancer Cell Line Cal-27.

doi:10.13701/j.cnki.kqyxyj.2018.12.008

Abstract

Abstract: Objective: To study the effects of thapsigargin on the viability and apoptosis of CAL-27 cells.Methods: CAL-27 cells were treated with different concentrations of thapsigargin and detected by methyl thiazolyl tetrazolium (MTT).After double staining with Annexin V-FITC/PI, the proportion of apoptotic cells was detected by flow cytometry.Western blotting was used to detect the activation of Caspase-3 and other apoptosis-related proteins.Results: Different concentrations of thapsigargin could inhibit the growth of CAL-27 cells in a dose-and dependent manner.The IC₅₀ was 15.09 μmol/L.DAPI staining showed that the nucleus becomes smaller and the chromatin shrinks with increasing doses of thapsigargin.Flow cytometry results showed that the apoptotic cells at late phase were increased.When detecting the apoptosis related proteins, the activation of Caspase-3 and Caspase-9 was shown after treatment with thapsigargin.Conclusion: Thapsigargin can significantly inhibit the proliferation of CAL-27 cells and induce the apoptosis of CAL-27 cells.The mechanism of action is related to the activation of Caspase-3 and Caspase-9.

Key words: Thapsigargin, Oral cancer, Apoptosis

DAI Qiao-qun, CHEN Wen-ying, SHI Fang-ping, CHEN Yu-lu. Experimental Study on Thapsigargin Induced Apoptosis in Tongue Cancer Cell Line Cal-27.[J]. Journal of Oral Science Research, 2018, 34(12): 1293-1296.

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