Effects of eIF4E-siRNA on Adhesion and Movement Ability of Tongue Squamous Cell Carcinoma Cells

doi:10.13701/j.cnki.kqyxyj.2019.01.012

Abstract

Abstract: Objective: To study the effect of eIF4E siRNA on adhesion and movement ability of tongue squamous cell carcinoma cells. Methods: Tongue squamous cell carcinoma cell line SCC25 was infected targeting eIF4E interferred lentivirus. The effect of silence was detected by qRT-PCR and Western blot. Cell proliferation and adhesion were detected by MTT assay. Cell motility was detected by scratch healing test. Cell invasion and migration were measured by Transwell chamber. The expression of N-cadherin, E-cadherin, and matrix degrading enzyme MMP-9 and MMP-2 proteins were detected by Western blot. Results: The levels of eIF4E mRNA and protein expression in SCC25 cells infected with eIF4E interfered with lentivirus were decreased. After silencing eIF4E, the proliferation activity of SCC25 cells was decreased, cell adhesion, cell motility, and the ability to invasion and migration were decreased, the level of E-cadherin protein in the epithelial marker was elevated, the level of N-cadherin protein in the interstitial marker was reduced, and the levels of MMP-9 and MMP-2 protein protein in the cells were decreased. Conclusion: eIF4E siRNA can downregulate the expression of eIF4E in tongue squamous cell carcinoma cells, and inhibit the adhesion, movement, invasion, and migration of tongue squamous cell carcinoma cells.

Key words: Tongue squamous cell carcinoma, Adhesion, Migration eIF4E

CHEN Chang, PENG Yong-jian, CHEN Yan. Effects of eIF4E-siRNA on Adhesion and Movement Ability of Tongue Squamous Cell Carcinoma Cells[J]. Journal of Oral Science Research, 2019, 35(1): 51-55.

References

[1] Bhat S, Kabekkodu SP, Jayaprakash C, et al. Gene promoter-associated CpG island hypermethylation in squamous cell carcinoma of the tongue [J]. Virchows Arch, 2017, 470(4):445-454.
[2] 冯铁军, 王玉栋, 潘宣,等. DEK基因的小干扰RNA转染对舌鳞状细胞癌细胞增殖和凋亡的影响[J].口腔医学研究,2018,34(4):414-418.
[3] 曲怡梅, 李亮亮, 徐宁. eIF4E生物学功能及其在肿瘤中作用研究进展[J].武警医学,2016,27(5):525-528.
[4] Sreevalsan S, Safe S. The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells [J]. Mol Cancer Ther, 2013, 12(11):2483-2493.
[5] Yoshizawa A, Fukuoka J, Shimizu S, et al. Overexpression of phospho-eIF4E is associated with survival through AKT pathway in non-small cell lung cancer [J]. Clin Cancer Res, 2009, 16(1):240-248.
[6] Dai D, Chen H, Tang J, et al. Inhibition of mTOR/eIF4E by anti-viral drug ribavirin effectively enhances the effects of paclitaxel in oral tongue squamous cell carcinoma [J]. Biochem Biophys Res Commun, 2016, 482(4):1259-1264.
[7] 周晓艳, 曾庆堂, 晁耐霞,等.eIF4E在肝癌组织中的表达及意义[J].山东医药,2012,52(7):38-40.
[8] Pashenkov MV, Balyasova LS, Dagil YA, et al. The Role of the p38-MNK-eIF4E Signaling Axis in TNF Production Downstream of the NOD1 Receptor [J].J Immunol, 2017, 198(4):1638-1648.
[9] Miras M, Truniger V, Querol-Audi J, et al. Analysis of the interacting partners eIF4F and 3'-CITE required for Melon necrotic spot virus cap-independent translation[J].Mol Plant Pathol , 2016, 18(5):635-648.
[10] 王晓丽,何跃东,黄娟,等.eIF4E和VEGF在宫颈癌中的表达及意义[J].四川大学学报(医学版),2010,41(1):179-181.
[11] 赵世巧,陈忠余,邓玮,等.eIF4E、CD44和E-cadherin在胃癌组织中的表达[J].广东医学,2016,37(2):230-232.
[12] Coleman LJ, Peter MB, Teall TJ, et al. Combined analysis of eIF4E and 4E-binding protein expression predicts breast cancer survival and estimates eIF4E activity [J]. Br J Cancer, 2009, 100(9):1393-1399.
[13] 雒静.eIF4E、FHIT、p53与舌鳞状细胞癌的相关性研究[D].中山大学,2009.
[14] Salehi Z, Mashayekhi F, Shahosseini F. Significance of eIF4E expression in skin squamous cell carcinoma [J]. Cell Biol Int , 2007, 31(11):1400-1404.
[15] Sforza V, Martinelli E, Ciardiello F, et al. Mechanisms of resistance to anti-epidermal growth factor receptor inhibitors in metastatic colorectal cancer [J]. World J Gastroenterol, 2016, 22(28):6345-6361.
[16] Che YL, Luo SJ, Li G, et al. The C3G/Rap1 pathway promotes secretion of MMP-2 and MMP-9 and is involved in serous ovarian cancer metastasis [J]. Cancer Let, 2015, 359(2):241-249.
[17] 张杨安,关为群,李群,等.口腔鳞状细胞癌组织中Periostin的表达及其在上皮间质转化中的作用[J].口腔医学研究,2017,33(3):262-265.