Effect of Cyclic-tension Strain on mRNA Levels of Leukemia Inhibitory Factor and Leukemia Inhibitory Factor Receptor in Human Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2019.09.011

Abstract

Abstract: Objective: To investigate the effects of cyclic-tension strain on the expression of LIF and LIFR in human periodontal ligament cells (HPDLC), and to illustrate the molecular mechanism underlying orthodontic periodontal tissue remodeling. Methods: In this study, HPDLC were subjected to 18% elongation by strain unit at 0.1Hz for 48h and 72h. The expressions of LIF, LIFR, and ALP mRNA were measured by real-time quantitative RT-PCR. Morphological changes were observed after mechanical stress. Results: Cells inclined parallel to each other and aligned their long axis perpendicular to the stretching force vector. The status was more and more transparent with extension of time. qRT-PCR showed that mRNA expression of LIF, LIFR, and ALP changed upon the stimulation. The expressions of LIF and LIFR were increased dramatically (P<0.01) and the expression of ALP was decreased significantly (P<0.01) after 72h. Conclusion: LIF and LIFR may play an role in periodontal tissue remodeling induced by orthodontic therapy.

Key words: human periodontal ligament cells, cyclic-tension strain, leukemia inhibitory factor

LIANG Youde, WEI Wei, XUE Weiwei, ZHOU Ruiping, FU Runying, WANG Yining. Effect of Cyclic-tension Strain on mRNA Levels of Leukemia Inhibitory Factor and Leukemia Inhibitory Factor Receptor in Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2019, 35(9): 863-867.

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