Abstract: Objective: To investigate the effects of different storage methods on adhesion and proliferation rate of osteoblasts on the surface of titanium implant. Methods: The titanium plates were modified with sandblasted and acid-etching technology and divided into three groups according to the storage methods. Group A was in the airtight container, group B was in the steaming water (ddH2O), and group C was in the sodium hydroxide (NaOH) solution for 4 weeks. Adhesion and proliferation of osteoblasts at different time points on the surface of titanium were evaluated by MTT assay, alkaline phosphatase activity assay, and laser scanning confocal microscope. Results: After 1 hour and 3 hours culture, the number of cells on group B and group C surface were higher than that on group A (P<0.05). After 1, 4, 7 days culture, the number of cells on group C was higher than those on other two groups (P<0.05). Under laser scanning confocal microscope, osteoblasts were found to spread better on group C surface. After 7 days culture, alkaline phosphatase activity of cells on group C was higher than those on group B and group A (P<0.05). Conclusion: The titanium plates preserved in the liquid was beneficial to maintain the biological activity. Sodium hydroxide solution might be the optimal way for implant storage.