Abstract: Objective: To prepare siRNA loaded collagen/bioglass composites and to investigate the function of collagen/bioactive glass composites, inducing local osteogenesis as well as sustained resealing siRNA. Methods: The loaded siRNA collagen/bioglass composites were prepared by freeze-drying method. The scaffold material characterization, mechanical properties, and degradation rate were evaluated. Micro CT was used to observe the adhesion and growth of MC3T3-E1 in scaffolds. The q-PCR was conducted to detect the activity of siRNA released from the composites. Results: The siRNA loaded collagen/bioglass composites was sponge-like material. Scanning electron microscopy showed that the bioactive glass was evenly dispersed in the collagen material. Compressive strength was low and porosity was appropriate. After the first week, the degradation rate was 30.7%. Cells form pseudopods on the material, tightly adhered to the surface of the material. Compared to the control group, the expression of noggin in the experimental group decreased by 16% (P <0.05), the difference was statistically significant. Conclusion: The siRNA loaded collagen/bioglass composite material prepared by freeze-drying composites has good performance and biocompatibility. As the scaffold degraded, siRNA released from the composites, and siRNA noggin remained partly active.

Key words: siRNA, Collagen, Bioglass