Spliced XBP1 Regulates Autophagy in Inflamed Odontoblasts

doi:10.13701/j.cnki.kqyxyj.2021.05.009

Abstract

Abstract: Objective: To examine the function of XBP1s in inflammation-induced autophagy in odontoblasts. Methods: Immunohistochemistry was used to examine the expression of XBP1 and autophagy markers (LC3 and ATG5) in human dental pulp samples. mDPC6T cells were stimulated by lipopolysaccharide (LPS) to establish an in vitro inflammatory model. The cells were further treated with the inhibitor KIRA6 and the reactive oxygen species (ROS) scavenging agent NAC, respectively. Immunofluorescence and western blotting were used to detect the expression of XBP1s and autophagy-related markers (LC3 and ATG5). Results: The expressions of XBP1, autophagy markers, and apoptosis markers were upregulated in odontoblast layers of human pulpitis tissues. LPS could induce the expression of spliced XBP1 in odontoblasts, and the expression of spliced XBP1 correlated with the expression of autophagy regulators under severe LPS stimulation. Moreover, the suppression of spliced XBP1 could reduce autophagy and apoptosis in inflamed odontoblasts. In addition, a reactive oxygen species (ROS) scavenger decreased the level of spliced XBP1 in inflamed mDPC6T cells. Conclusion: Spliced XBP1 can regulate autophagy in inflamed odontoblasts

Key words: XBP1s, autophagy, odontoblasts, inflammation

WANG Yanjie, WANG Haisheng, GUO Weiting, ZHANG Lu. Spliced XBP1 Regulates Autophagy in Inflamed Odontoblasts[J]. Journal of Oral Science Research, 2021, 37(5): 418-424.

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