Mechanism of Interleukin-17 Regulating Matrix Metalloproteinase Expression and Inflammation in Dental Pulp

doi:10.13701/j.cnki.kqyxyj.2021.05.010

Abstract

Abstract: Objective: To investigate the role of interleukin-17 in dental pulp inflammation and the relationship between IL-17 and MMPs. Methods: Immunohistochemical staining was used to detect the expression of TNF-α and IL-17 in pulp tissues. TNF-α or IL-17 recombinant protein was used to treat human dental pulp cells. RT-qPCR, Western blotting, and ELISA were used to detect the expression of mRNA and protein levels of IL-17, MMP-1, MMP-2, MMP-3 and MMP-13. Gelatin zymography and collagen zymography were used to detect the enzymatic activity of MMP-1 and MMP-2. Results: IL-17 was high-expressed during pulpitis. TNF-α treatment could induce expression of IL-17 in HDPCs. IL-17 mainly contributed to its positive regulatory role in inflammation through up regulating MMPs and activating protein kinase MAPK pathway. Conclusion: IL-17 acts as an inflammatory mediator in dental pulp inflammation by acting MAPK pathway. IL-17 can induce the expression of MMP-1 and MMP-2 in HDPCs

Key words: pulpitis, interleukin-17, matrix metalloproteinase, immunology

LIU Mengyu. Mechanism of Interleukin-17 Regulating Matrix Metalloproteinase Expression and Inflammation in Dental Pulp[J]. Journal of Oral Science Research, 2021, 37(5): 425-430.

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