Apoptosis Promoting Effect of Icariin on Human Oral Squamous Cell Carcinoma Cells by Regulating Ki-67 and Notch-1 Gene Expression

doi:10.13701/j.cnki.kqyxyj.2021.06.006

Abstract

Abstract: Objective: To explore the effect of icaritin (ICT) on the apoptosis of oral squamous cell carcinoma cells-9 (SCC-9) through regulating Ki-67 and Notch-1 gene expression, and to provide experimental basis for ICT treatment of oral squamous cell carcinoma. Methods: SCC-9 cells were treated with 4, 8, 16, 32, and 64 μmol/L ICT respesctively, and equal volume of culture medium was added as blank control group. CCK-8 method was used to detect the cell survival rate. Clone formation test was used to detect the capability of cell clones. Flow cytometry was used to detect the degree of apoptosis,and real-time quantitative PCR was used to detect the mRNA expression levels of Ki-67 and Notch-1. Results: The proliferation rate and clone number of SCC-9 cells treated with ICT were significantly lower than those of control group (P<0.01). The expression levels of Ki-67 and Notch-1 in SCC-9 cells treated with ICT were significantly lower than those of control group (P<0.01). Conclusion: ICT can influence the proliferation and promote apoptosis of SCC-9 cells by down regulating the gene expression of Ki-67 and Notch-1.

Key words: ICT, Ki-67/Notch-1, squamous cell carcinoma, apoptosis, proliferation

WANG Yixue, XU Yingjie, CONG Beibei, WEI Xin. Apoptosis Promoting Effect of Icariin on Human Oral Squamous Cell Carcinoma Cells by Regulating Ki-67 and Notch-1 Gene Expression[J]. Journal of Oral Science Research, 2021, 37(6): 505-508.

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