Effect of Conditional Knockout of Fam20C on Expression of MMP-1 and TIMP-1 in Periodontal Ligament of Mice

doi:10.13701/j.cnki.kqyxyj.2022.05.007

Abstract

Abstract: Objective: To investigate the effect of knocking down Fam20C driven by the 3.6 kb Col1α1 promoter on periodontal tissues in mice. Methods: Paraffin sections of the mandibular molar region were routinely made from paraffin blocks of mandibular tissues of 3.6 kb Col1α1-Cre; Fam20C^fl/fl mice and normal control mice in the same litter at 4 and 12 weeks of age. The paraffin sections were used for HE staining, Masson staining, and immunohistochemical staining. The histological changes of cementum, alveolar bone, and periodontal ligament in the molar region of mice in all groups were observed. Optical density values were measured to analyze the differential expression of MMP-1 and TIMP-1 in the periodontal ligament. Results: Compared with normal control mice, the cellular cementum in the molar area of 4-week-old Fam20C knockout mice became thinner; The height of alveolar bone between molars was reduced. The diameter of collagen fiber bundles in the periodontal ligament was decreased, and some fiber bundles were loose and disordered. The positive expression of MMP-1 in periodontal ligament was significantly increased (P<0.05). The positive expression of TIMP-1 was slightly decreased (P>0.05). The MMP-1/TIMP-1 ratio was significantly increased (P<0.05). 12-week-old Fam20C knockout mice had more severe periodontal tissue lesions than 4-week-old knockout mice. Their cellular cementum was significantly decreased. The height of alveolar bone between molars was significantly reduced. The collagen fiber bundles of the periodontal ligament were disorganized. They were slenderer and sparse. A large number of collagen fiber bundles below the root neck were broken and detached from the surface of cementum and alveolar bone. The trend of differential expression of MMP-1 and TIMP-1 in the periodontal ligament was consistent with the 4-week-old group. Conclusion: The knockout of Fam20C driven by the 3.6 kb Col1α1 promoter may lead to periodontal lesions in mice by up-regulating the MMP-1/TIMP-1 ratio in the periodontal ligament.

Key words: Fam20C, conditional knockout, periodontal ligament, MMP-1, TIMP-1

YUAN Zhaoyang, WU Guodong, QI Hao, MA Su, LIU Peihong. Effect of Conditional Knockout of Fam20C on Expression of MMP-1 and TIMP-1 in Periodontal Ligament of Mice[J]. Journal of Oral Science Research, 2022, 38(5): 417-423.

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