Static Orthodontic Pressure Regulates the Expression of MMP-2 and MMP-9 in Periodontal Ligament Cells via p38MAPK Pathway

doi:10.13701/j.cnki.kqyxyj.2021.04.010

Abstract

Abstract: Objective: To instigate the regulation effects of static pressure on the expression of MMP-2 and MMP-9 in periodontal ligament cells (PDLCs) and to explore related pathways. Methods: PDLCs were cultured in vitro. Cells of 4-7 generations were randomly divided into 5 groups, i.e. 0, 2 g/cm²(24 h), 2 g/cm²(48 h), 4 g/cm²(24 h), and 4 g/cm²(48 h) groups. Continuous static pressure was used to detect cell morphology and skeleton F-actin. The effect of continuous static pressure on the expression of MMP-2, MMP-8, and MMP-9 was observed by western blot. Related MAPK pathways including JNK, ERK, and p38MAPK in the static pressure induced expression of MMPs were studied by western blotting. p38MAPK blocker SB203580 was added into each groups, and the effect of p38MAPK was observed by western blot and immunofluorescence. Results: Under different continuous static pressures, there was no obvious change in the morphology of PCLCs. F-actin staining showed no significant difference in cytoskeleton. Western blotting showed that static pressure had no significant effect on the expression of MMP-8, and the expressions of MMP-2 and MMP-9 were significantly up-regulated. This upregulation was related to the p38MAPK pathway, but not to the JNK and ERK pathways. Conclusion: Continuous static pressure regulates the expression of MMP-2 and MMP-9 in periodontal ligament cells. This regulation is related to the p38MAPK pathway but JNK and ERK pathways.

Key words: static pressure, periodontal ligament cells, MMP-2, MMP-9

ZHANG Xun, LIN Yuanyuan, TIAN Qunli. Static Orthodontic Pressure Regulates the Expression of MMP-2 and MMP-9 in Periodontal Ligament Cells via p38MAPK Pathway[J]. Journal of Oral Science Research, 2021, 37(4): 319-324.

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