Preliminary Study of Demineralized Dentin Matrix- Platelet-rich Plasma Gel Applying to Scaffold Material of Periodontal Bone Tissue Engineering.

Abstract

Abstract: Objective: To evaluate the effects of demineralized dentin matrix (DDM) to the platelet-rich plasma (PRP) gel delivery system for growth factors. Methods: After double centrifugation for preparation of PRP, the concentrations of platelet-derived growth factor (PDGF) and transforming growth factor-β (TGF-β) in the whole blood gel (group C), PRP gel (group B), and PRP/DDM gel (group A) were measured in two weeks by enzyme-linked immunosorbent assay (ELISA). The structure of prepared PRP/DDM gel was observed by scanning electron microscopy (SEM). DDM was implanted into the pit of femoral muscles of SD rats and tissue sections were performed to study the toxicity reaction. Results: Compared with other two groups, PRP/DDM had a much longer time to continuously release the growth factors (P<0.05). Through SEM and tissue section, it was found that PRP and DDM combined with each other closely and orderly and DDM had a fine biocompatibility. Conclusion: The combination of PRP and DDM can better slower the release of growth factors.

CLC Number:

R781.4

HOU Zong-peng, GUO Liang-wei, ZHOU Jin, Sun Jin-huan.. Preliminary Study of Demineralized Dentin Matrix- Platelet-rich Plasma Gel Applying to Scaffold Material of Periodontal Bone Tissue Engineering.[J]. Journal of Oral Science Research, 2015, 31(12): 1184-1187.

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