Silencing ITGA6 Promotes Odontogenic Differentiation of hDPSCs Through Activating FOXO1 Signaling Pathway

doi:10.13701/j.cnki.kqyxyj.2022.10.006

Abstract

Abstract: Objective: To investigate whether silencing integrin α6 (ITGA6) affects odontogenic differentiation of human dental pulp stem cells (hDPSCs) through activating FOXO signaling pathway. Methods: hDPSCs were transfected with ITGA6 silencing lentiviruses constructed by our research group, real-time PCR and Western blot were applied to evaluate the interference efficiency of ITGA6 silencing lentiviruses. Meanwhile, FOXO signaling pathway activity was detected in empty vector group (sh-NC group) and ITGA6 silencing group (sh-ITGA6 group). FOXO1 was inhibited by AS1842856. Western blot and alkaline phosphatase (ALP) staining were performed after 7 days of mineralization induction to observe the odontogenic differentiation of hDPSCs. Results: ITGA6 silencing lentiviruses effectively inhibited the expression of ITGA6 in hDPSCs. Compared with sh-NC group, the mRNA expression of FOXO1 was upregulated (P<0.05) and Western blot results showed that the expression of FOXO1 in nuclear was increased (P<0.05) in sh-ITGA6 group. The odontogenic differentiation markers DSPP (dental sialophosphoprotein) and DMP-1 (dental matrix protein-1) were upregulated in sh-ITGA6 group assessed by Western blot, while the expression of DSPP and DMP-1 were decreased after sh-ITGA6 treated by AS1842856 (P<0.05). ALP staining results showed that the ALP staining in sh-ITGA6 group was significantly deeper, however, after treated sh-ITGA6 with AS1842856, ALP staining became shallower. Conclusion: Silencing ITGA6 promoted odontogenic differentiation of hDPSCs through activating FOXO1 signaling pathway.

Key words: integrin α6, dental pulp stem cells, FOXO1 signaling pathway, odontogenic differentiation

WANG Kaili, ZHANG Weiwei, LI Yanping, HE Lina, ZHANG Shuang, PAN Shuang, NIU Yumei. Silencing ITGA6 Promotes Odontogenic Differentiation of hDPSCs Through Activating FOXO1 Signaling Pathway[J]. Journal of Oral Science Research, 2022, 38(10): 930-935.

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