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28 October 2022, Volume 38 Issue 10 Previous Issue    Next Issue

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Biological Insights into Orthodontically Induced Root Resorption HE Hong, DU Mingyuan 2022, 38(10): 907-912.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.001 Abstract ( 251 )   PDF (1148KB) ( 325 )   Orthodontically induced root resorption is a common complication caused by orthodontic force, which is mainly characterized by the destruction of cementum and other dental tissues. It is mainly caused by local aseptic inflammation induced by orthodontic force and regulated by multiple cells and related molecules. Orthodontically induced root resorption is highly concerned by orthodontists due to its high incidence and potential impact on the health of periodontal tissues. In this article, biological characteristics of the progress of orthodontically induced root resorption, the main involved cells, related regulatory molecules, and cementum repair are described. References | Related Articles | Metrics

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Research Advances in Effects of Osteoporosis and Related Therapeutic Agents on Orthodontic Treatment LIU Xinyu, HU Min 2022, 38(10): 913-916.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.002 Abstract ( 263 )   PDF (795KB) ( 356 )   With the increase of osteoporosis year by year, the proportion of orthodontic patients suffering from osteoporosis is also increasing. The normal bone metabolism of osteoporosis patients is destroyed, which also influences the orthodontic tooth movement based on the reconstruction of periodontal tissue. Due to the drugs treating osteoporosis affect bone remodeling, they may affect the alveolar bone. How to rebuild the bone remodeling balance of alveolar bone while ensuring the efficiency of orthodontic treatment should be the focus of future research. References | Related Articles | Metrics

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Application of Optical Coherence Tomography in Oral Mucosal Diseases ZHANG Chang, LIU Yang 2022, 38(10): 917-921.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.003 Abstract ( 278 )   PDF (961KB) ( 458 )   Optical coherence tomography (OCT) provides a real-time and non-invasive method to visualize layered structures with interferometric tomography. It is widely used in ophthalmology and cardiovascular fields. In recent years, OCT has emerged in the non-invasive diagnosis of oral mucosal diseases. This article explains the principles, classification, and application of OCT, focusing on the application of OCT in the oral mucosal diseases to provide references for further research and clinical applications. References | Related Articles | Metrics

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Challenges and Countermeasures of Pulp Regeneration Technology in Mature Permanent Teeth HUANG Mengyu, LIU Shengbo 2022, 38(10): 922-925.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.004 Abstract ( 265 )   PDF (792KB) ( 331 )   Pulp regeneration technology is becoming a research hot issue in recent years, and it is also a good alternative treatment for root canal treatment. The current researches on pulp regeneration mainly focused on immature permanent teeth. In fact, there are far more mature permanent teeth that require endodontic treatment than immature permanent teeth. Therefore, pulp regeneration has great application prospects for mature permanent teeth. Compared with immature permanent teeth, mature permanent teeth have difficulties such as fully developed apical foramen, diverse anatomical structures, and complex microenvironment, etc. It is difficult to achieve pulp regeneration. This article reviews the related researches in recent years and discusses the difficulties and coping solutions of mature permanent teeth in pulp regeneration, in order to provide reference for the future development of pulp regeneration technology in mature permanent teeth. References | Related Articles | Metrics

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Application and Prospect of Microfluidics in the Field of Periodontics LIU Enyan, DING Yi 2022, 38(10): 926-929.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.005 Abstract ( 232 )   PDF (797KB) ( 220 )   Microfluidics is a kind of device, which uses analytic biology and chemistry as the research object, integrates sample preparation, reaction, separation, and detection into a piece of a few square centimeters chip, forms network by micro channel, runs through the system with controllable fluid, and replaces the test of conventional biochemical laboratory. In recent years, the device has gradually become a hot research issue in the diagnosis and treatment of periodontal disease, mainly related to periodontal pathogens and protein biomarkers detection, and drug research and development of periodontal therapy. A large number of studies have shown that microfluidics plays an important role in basic research and clinical research in the field of periodontics. In addition, it has great potential to replace traditional biological laboratories and become a novel periodontal disease research method in the future. Starting from the related concepts and technologies of microfluidics, this paper reviews the application of microfluidics in the diagnosis and treatment of periodontal disease, and looks forward to its possible application directions in the periodontal field in the future, so as to provide experimental ideas and references for researchers in related fields. References | Related Articles | Metrics

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Silencing ITGA6 Promotes Odontogenic Differentiation of hDPSCs Through Activating FOXO1 Signaling Pathway WANG Kaili, ZHANG Weiwei, LI Yanping, HE Lina, ZHANG Shuang, PAN Shuang, NIU Yumei 2022, 38(10): 930-935.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.006 Abstract ( 199 )   PDF (1931KB) ( 220 )   Objective: To investigate whether silencing integrin α6 (ITGA6) affects odontogenic differentiation of human dental pulp stem cells (hDPSCs) through activating FOXO signaling pathway. Methods: hDPSCs were transfected with ITGA6 silencing lentiviruses constructed by our research group, real-time PCR and Western blot were applied to evaluate the interference efficiency of ITGA6 silencing lentiviruses. Meanwhile, FOXO signaling pathway activity was detected in empty vector group (sh-NC group) and ITGA6 silencing group (sh-ITGA6 group). FOXO1 was inhibited by AS1842856. Western blot and alkaline phosphatase (ALP) staining were performed after 7 days of mineralization induction to observe the odontogenic differentiation of hDPSCs. Results: ITGA6 silencing lentiviruses effectively inhibited the expression of ITGA6 in hDPSCs. Compared with sh-NC group, the mRNA expression of FOXO1 was upregulated (P<0.05) and Western blot results showed that the expression of FOXO1 in nuclear was increased (P<0.05) in sh-ITGA6 group. The odontogenic differentiation markers DSPP (dental sialophosphoprotein) and DMP-1 (dental matrix protein-1) were upregulated in sh-ITGA6 group assessed by Western blot, while the expression of DSPP and DMP-1 were decreased after sh-ITGA6 treated by AS1842856 (P<0.05). ALP staining results showed that the ALP staining in sh-ITGA6 group was significantly deeper, however, after treated sh-ITGA6 with AS1842856, ALP staining became shallower. Conclusion: Silencing ITGA6 promoted odontogenic differentiation of hDPSCs through activating FOXO1 signaling pathway. References | Related Articles | Metrics

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In Vitro Study of Root Canal Sealers iRoot SP and AH Plus on Adhesive Properties of Dentin REN Yingchao, KONG Xianghong, HE Miaomiao, ZHANG Mengya, XIAO Yan 2022, 38(10): 936-940.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.007 Abstract ( 248 )   PDF (866KB) ( 291 )   Objective: To study the effect of iRoot SP and AH Plus on the bonding strength of root inner canal wall. Methods: Sixty single tube teeth were collected and randomly divided into iRoot SP and AH plus (n=30). Each group was randomly divided into 3 subgroups and filled by warm vertical technique, the single cone technique, and lateral condensation technique. After 1 week, all samples were cut into slices with 1 mm in thickness. The strength was calculated by universal test machine, and the fracture mode and the percentage were calculated. Results: iRoot SP and AH plus were similar and the difference was not significant (P>0.05). The highest value (8.38±3.91) MPa was in the apical region of iRoot SP (P<0.05). The proportion of fracture mode in each group was: mixed failure>cohesin failure>adhesion failure. Conclusion: iRoot SP can be similar or even better than AH plus, and the apical region has the highest bonding strength by the single cone method. The fracture mode is dominated by mixed failure. References | Related Articles | Metrics

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Effect of "Apical Induction Combined with Apical Barrier Therapy" on Adult Patients with Open Apical Root and Chronic Apical Periapical Inflammation LIU Ting, HUANG Yequan, YANG Weidong, WANG Wenmei, ZHU Yanan 2022, 38(10): 941-945.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.008 Abstract ( 201 )   PDF (929KB) ( 258 )   Objective: To evaluate the clinical efficacy of "apical induction combined with apical barrier therapy" in the treatment of adult patients with open apical root and chronic apical periapical inflammation. Methods: Sixty patients with open apical root and chronic periapical periodontitis who were admitted to our hospital were selected and randomly divided into the experimental group and control group. The experimental group of 15 teeth were treated with apical induction, ie, vitapex continuous sealing was used. And iRoot BP Plus apical barrier was performed after calcification barrier was formed in the apical tip. In the control group, 15 patients received conventional iRoot BP Plus apical barrier surgery, and the postoperative old-periapical index (O-PAI), operation time of 1/3 lower root canal segment, and average efficacy were observed in the two groups. Results: Fifteen patients had correct-filling in the experimental group, while in the control group, 10 patients had correct-filling and 5 patients had overfilling. The O-PAI and periapical lesion area of experimental group 12 months after operation was lower than that of control group (P<0.05). The operation time of 1/3 lower root canal segment in the experimental group was shorter than that in the control group (P<0.05). The success rate of 12 months’ follow-up in the experimental group was higher than that in the control group (P<0.05). Conclusion: The application of "apical induction combined with apical barrier" in the treatment of adult open chronic periapical periodontitis improved the O-PAI, periapical lesion area, and success rate for 12 months, and the operation was relatively simple, which can be used for clinical promotion. References | Related Articles | Metrics

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Correlation Analysis of DLX2 Gene Polymorphism and Dental Fluorosis ZAN Binbin, CHEN Liming 2022, 38(10): 946-949.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.009 Abstract ( 138 )   PDF (820KB) ( 127 )   Objective: To observe the distribution of DLX2 gene polymorphism in the population with dental fluorosis caused by coal-burning pollution in Bijie city, and to explore the relationship between genetic susceptibility and tolerance of dental fluorosis and DLX2 gene polymorphism. Methods: Under a case-control study, 117 dental fluorosis patients (experimental group) and 108 non-dental fluorosis subjects (internal control group) were enrolled in Bijie city (fluorosis area), and 115 non-dental fluorosis subjects (external control group) were enrolled in Guiyang two urban areas (non-epidemic area). Their remaining peripheral blood samples after routine blood test were collected. The genomic DNA from blood samples were extracted. SNP loci was screened for PCR amplification, and the amplification products were used Sanger sequencing technology to detect the single nucleotide polymorphism sites' genotypes. The differences of different genotypes and allele frequency distribution among groups were analyzed. Results: There was no significant difference in genotype and allele frequency distribution of rs743605 loci among groups (P>0.05). The frequency of CC genotype was significantly higher in severe dental fluorosis than that in mild and moderate dental fluorosis. The frequency of T allele in severe dental fluorosis was significantly lower than that in mild and moderate dental fluorosis, with difference statistically significant (P<0.05). The distribution of rs3762499 genotype was significantly different among groups (P<0.05). The AG genotype frequency in the internal control group was significantly higher than that in the experimental group (P<0.05). There was no significant difference in genotype and allele frequency distribution among mild, moderate, and severe dental fluorosis (P>0.05). Conclusion: The polymorphism of rs743605 and rs3762499 in DLX2 gene is correlated with dental fluorosis. References | Related Articles | Metrics

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Comparative Study of Tooth Preparation for Veneer by Electric Handpiece and Air-turbine Handpiece LUO Bizhu, CHEN Yingming, YIN Lu, XU Qiusheng, YANG Changwei, LIANG Yue 2022, 38(10): 950-953.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.010 Abstract ( 249 )   PDF (1353KB) ( 240 )   Objective: To compare the surface roughness, the degree of microleakage, and fracture resistance of veneers after using air-turbine handpiece and electric handpiece at different speeds to prepare tooth in vitro. Methods: Sixty premolars without decay were extracted and divided into 4 groups (n=15) according to the way of preparation. Air-turbine handpiece (Group A) and electric handpiece with 200,000 r/min (Group B), 50,000 r/min (Group C), and 20,000 r/min (Group D) were used to prepare tooth in vitro. White light interferometer was used to measure the surface roughness. The microleakage of the mid-longitudinal section was surveyed under a microscope after bonding veneers with resin cement and simulated for aging. The fracture resistance was measured by an electronic universal testing machine. Results: There was no significant difference on the surface roughness and fracture resistance between four groups (P>0.05). However, there was significant difference in the microleakage degree after aging test (P<0.05). Conclusion: The surface roughness and fracture resistance of veneers are not affected by the type and speed of handpieces. Electric handpiece at 200,000 r/min had the smallest average microleakage of veneers. References | Related Articles | Metrics

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Three-dimensional Finite Element Analysis of Maxillary Central Incisors Restored with Three Kinds of Personalized Custom Post-and-core with Angles GUO Xiaoyang, CHEN Zhiyu, FU Yixuan, LI Shaoping, Gao Maomao 2022, 38(10): 954-958.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.011 Abstract ( 209 )   PDF (1217KB) ( 204 )   Objective: To analyze the stress distribution characteristics of different materials custom post-and-core at different angles for maxillary central incisor by three-dimensional finite element analysis. Methods: The models with different inclination angles of post-and-core-and-crown in the maxillary central incisor were created. Three post-and-core materials were used in the model: cast gold-palladium alloy, glass fiber reinforced resin, and carbon fiber reinforced polyether ether ketone (CFR-PEEK). They were finally restored with an all-ceramic crown. The equivalent stresses in the post-and-core and dentin of the different models were analyzed under centric occlusion. Results: At the same angle, the highest stresses in the cast gold-palladium post-and-core correspond to the lowest stresses in the dentin. The CFR-PEEK post-and-core exhibited the lowest equivalent stress, corresponding to the highest stress in dentin. The stress of post-and-core and dentin decreases with increasing buccal angle for the same material. Conclusion: The angle of the post-and-core had no significant effect on restoration and dentin resistance under the same loading direction. The post-and-core with a lower elastic modulus is recommended when the dentin ferrule is intact. References | Related Articles | Metrics

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Preliminary Exploration of Artificial Intelligence in Diagnosis of Displaced Root Fracture Based on Convolutional Neural Network HU Yanni, CAO Dantong, WANG Baixin, CHEN Yin, LIN Zitong 2022, 38(10): 959-962.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.012 Abstract ( 158 )   PDF (1190KB) ( 155 )   Objective: To explore the value of convolutional neural network in the diagnosis of displaced root fracture (DRF) based on cone-beam CT (CBCT). Methods: The CBCT images of 286 patients with DRF were collected in our hospital. The regions of interest were obtained by automatic and manual cropping, and labeled as positive or negative manually. ResNet 50 was used for training and establishing diagnosis models for DRF. The diagnosis efficiency of two models was calculated (accuracy, specificity, and sensitivity). Results: One hundred and sixty-four patients' images were used for automatic cropping and 122 patients' images were used for manual cropping. In the automatic cropping group, there were 184 positive images and 186 negative images in the training set; 64 positive images and 66 negative images in the test set. While in the manual cropping group, there were 96 positive images and 114 negative images in the training set; 32 positive images and 36 negative images in the test set. The accuracy, specificity, and sensitivity were 98.5%, 100%, and 96.9% with manual cropping; while they were 90.0%, 95.5%, and 84.4% with automatic cropping. Conclusion: Convolutional neural network ResNet 50 could be used to detect displaced root fractures with high diagnosis efficiency. References | Related Articles | Metrics

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Application Value of MDCT Enhancement Combined with CTA Multidimensional Technology in Maxillofacial Soft Tissue Hypervascular Tumors ZHANG Wenxin, SHAN Shan, WANG Tiemei, LIU Shu, HUANG Xiaofeng, WANG Yuxin 2022, 38(10): 963-967.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.013 Abstract ( 155 )   PDF (1530KB) ( 125 )   Objective: To evaluate the clinical imaging features of oral and maxillofacial hypervascular tumors in oral and maxillofacial soft tissue by MDCT enhancement combined with CTA multi-dimensional technique. Methods: The degree of blood supply and indexes of 21 cases of hypervascular tumors were retrospectively analyzed, and the pathological results were used as the diagnostic gold standard to evaluate the sensitivity and specificity of MDCT plain scan and enhanced CTA multidimensional technology in the diagnosis of oral and maxillofacial soft tissue hypervascular tumors. The ROC curve was drawn to analyze and evaluate the efficacy. Results: Among 21 patients, the diagnostic accuracy of MDCT enhancement combined with CTA multidimensional technology was 90.48%, the AUC of venous phase CT value was 0.80, the sensitivity was 83.30%, and the specificity was 72.73%. Conclusion: MDCT enhancement combined with CTA multi-dimensional technology can be used to evaluate the blood supply of maxillofacial soft tissue hypervascular tumors before operation, and the CT value in the venous phase of tumors has the highest evaluation efficiency, which can reduce the risk of blood loss during maxillofacial hypervascular tumors. The preoperative non-invasive assessment of tumor blood supply has certain guiding significance for the formulation of clinical treatment plans. References | Related Articles | Metrics

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External Root Resorption of Second Molar Associated with Mesially Impacted Mandibular Third Molar Based on CBCT YU Tianping, WANG Rong, YANG Bin 2022, 38(10): 968-971.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.014 Abstract ( 181 )   PDF (1317KB) ( 160 )   Objective: To analyze the relationship between mandibular third molar (MTM) and external root resorption (ERR) of mandibular second molar by CBCT. Methods: CBCT scans involving 302 MTMs were assessed. The patient gender and age, mesial angulation and impaction depths of MTMs, and MTMMCEJ-MSMDCEJ distances and contact point localization were assessed. The relationship between each factor and the ERR was analyzed. Results: The detection rate of ERR in second molars was 15.89%. Age, the angle of occlusion between MTM and mandibular second molar, and MTMMCEJ-MSMDCEJ were identified as risk factors for ERR. When the age≥35 years, the angle of occlusion was 45°-60°, and MTMMCEJ-MSMDCEJ＞6 mm, ERR was significantly increased. Conclusion: The age (≥35 years), the angle of occlusion 45°-60°, and MTMMCEJ-MSMDCEJ (>6 mm) could be regarded as the guidelines for preventive removal of MTM. References | Related Articles | Metrics

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Black Triangle in Orthodontic Patients with Mandibular Incisor Extractions: Prevalence and Etiology ZHU Meng, SUN Weiman, WANG Jinmeng, LI Houxuan, LEI Lang 2022, 38(10): 972-976.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.015 Abstract ( 382 )   PDF (1010KB) ( 291 )   Objective: To explore the incidence and influencing factors of black triangle in patients with single mandibular incisor extractions after orthodontic treatment. Methods: Eighty fixed orthodontic treatment patients who had one mandibular incisor extracted were enrolled in this retrospective study, and were divided retrospectively into the normal group and the black triangle group based on whether there was a black triangle between the adjacent teeth of the extracted tooth in the posttreatment intraoral frontal photos. According to the intraoral photos, digital models, panoramic radiographs, and other clinical data, the position of interdental contact point, root angle, distance from interdental contact point to alveolar bone crest, width and area of embrasure space, and other clinical parameters were measured and analyzed. Results: After extraction of one mandibular incisor, the incidence of black triangle was 72.5%, with mild incidence of 57.5%, moderate incidence of 15%, and no severe black triangle occurred. There were statistically significant differences between the normal group and the black triangle group in age, position of interdental contact point, root angle, distance from interdental contact point to alveolar bone crest, and width and area of embrasure space. Logistic regression analysis showed that the adjacent teeth root angle and the distance from interdental contact point to alveolar bone crest were the main risk factors for the black triangle after fixed orthodontic treatment in patients with mandibular incisor extraction. Conclusion: The incidence of black triangle was higher in patients who had one mandibular incisor extracted. Orthodontic treatment needs to control the root angle of the adjacent teeth and maintain the health of periodontal tissue so as to reduce the occurrence of black triangle. References | Related Articles | Metrics

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Effect of Wearing Invisible Appliance on Vertical Height of Posterior Teeth HAN Yan, HU Yingchao, YANG Minzhi, ZHANG Jianan, LU Haiping, ZHANG Duanqiang 2022, 38(10): 977-980.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.016 Abstract ( 168 )   PDF (910KB) ( 184 )   Objective: To investigate the difference between eating with aligners (EWA) and eating without aligners (EWOA) on the vertical height of posterior teeth. Methods: A prospective study was performed on 60 subjects selected for treatment with invisible appliance, all of whom were treated without tooth extraction. The patients were divided into the experimental group and control group according to whether or not they wore the invisible appliance while eating, with 30 cases in the experimental group and 30 cases in the control group. The experimental group was suggested to wear the invisible appliance to eat, while the control group did not wear the invisible appliance to eat. The vertical displacement distance of the first maxillary molars (U6 and L6) and the bone changes of the upper and lower mandible were compared between two groups before and after treatment. Results: There were no significant differences between two groups in molar height, S-Go/N-Me (%), FMA(°), OP-FH(°), and MP-OP(°) before and after treatment (P>0.05). Conclusion: There was no effect on the vertical height of posterior teeth by wearing an invisible orthodontic device when eating. References | Related Articles | Metrics

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Clinical Analysis of 14 Cases of Ameloblastic Carcinoma YUAN Zhuang, LUO Hao, WU Kailiu, HE Jie, MENG Jian 2022, 38(10): 981-985.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.017 Abstract ( 208 )   PDF (940KB) ( 213 )   Objective: To explore the clinical characteristics, diagnosis, and treatment of ameloblastic carcinoma (AC) by analyzing clinical cases and reviewing the literature. Methods: The clinical data of 14 patients with AC were retrospectively analyzed. Results: There were 10 male and 4 female patients included in this study, with a male-to-female ratio of 2.5∶1. Their ages ranged from 15 to 71 years and the mean age was 40.9 years. Six patients' lesion location was maxilla and 8 patients' was mandible, and the maxilla-to-mandible ratio was 0.75∶1. All patients were subjected to surgical resection and followed-up for 0.5-7 years to record recurrence. There were 9 recurrences in the case series, of which 1 had cervical lymph node metastasis, and 2 had lung metastasis after surgery. Conclusion: AC is a rare neoplasm of the odontogenic epithelium. Secondary AC is mostly caused by repeated surgical intervention. Recurrence rate of AC is closely related to lesion location and treatment. At present, extended surgical resection is still the preferred treatment and postoperative long-time followed-up is necessary. References | Related Articles | Metrics

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Comparative Analysis on Formation Mechanism of Oral Microbial Diversity Between Smokers and Non-smokers KANG Jizhe, WANG Wei, YE Junjie, YANG Yang, DONG Chen 2022, 38(10): 986-990.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.018 Abstract ( 171 )   PDF (2021KB) ( 157 )   Objective: To explore the influence mechanism of tobacco on the formation and succession of oral microbial diversity. Methods: The specific growth rate, lipid content, and population number of Streptococcus, Prevotella, Haemophilus, Rothia, and Veillonella were investigated in the oral cavity of 20 smokers and 20 non-smokers for 4 months. Results: The intracellular lipid content of non-smoker oral microorganisms increased significantly, but the specific growth rate varied. Formation and maintain non-smoker oral microbial diversity included two basic dynamic mechanism, one was the substrate decomposition product negative feedback regulation of population, and the second was the delay response of microorganisms in oral environment. On the contrary, in smokers, the diversity of oral flora decreased and had a trend of disorder. Conclusion: Smoking has a negative effect on the diversity of oral microflora, which is difficult to form a stable population diversity. The number of each species in non-smokers oral microbial community showed asynchronous convergence fluctuations, which greatly weakened the interspecific competition within the oral microbiome and formed and maintained a higher species diversity. References | Related Articles | Metrics

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Questionnaire Study on Application of Rubber Dam Isolation in Hubei Province LI Shaolan, LIU Yang, LI Zhiyong 2022, 38(10): 991-994.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.019 Abstract ( 207 )   PDF (766KB) ( 351 )   Objective: To investigate the application status of rubber dam isolation in Hubei dental practice. Methods: A self-designed questionnaire of rubber dam isolation was distributed to 1171 dental practitioners in 13 regions of Hubei Province, covering public and private dental clinics. Results: A total of 1167 valid questionnaires were recovered. The total score rates in knowledge, attitude, and practice of rubber dam isolation were 76.4%, 51.3%, and 34.9%, respectively. The highest proportion of treatment items using rubber dam isolation was root canal therapy (81.4%), the highest proportion of treated teeth was mandibular posterior teeth (84.4%), and the average operation time for a single tooth was 3.2 minutes. 71.6% of the respondents used rubber dam isolation, and the average application rate was 32.4%. The application rate of rubber dam isolation was related to regions, institution levels, educational backgrounds, majors, average operation time for a single tooth, working years, and the charging price. The technical factor had the highest average comprehensive score affecting the application of rubber dam isolation, followed by the price factor. Among the measures to improve rubber dam isolation, the highest comprehensive score was the adjustment of charging price. Conclusion: The application of rubber dam isolation in Hubei dental practice needs to be improved. Measurements such as strengthening the training of rubber dam isolation and adjusting the charging standard can be taken to improve the application rate of rubber dam isolation. References | Related Articles | Metrics

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Juvenile Dentinogenic Ghost Cell Tumor: A Case Report YE Tingting, CHEN Weihua, PEI Jing, JIA Yunxiang, WU Haiying 2022, 38(10): 995-996.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.020 Abstract ( 281 )   PDF (1409KB) ( 222 )   Dentinogenic ghost cell tumour is a rare odontogenic tumor with local invasiveness. Clinical and imaging studies are not specific, especially when there are few tissues submitted for inspection or insufficient materials are obtained. It is easy to miss the diagnosis. A case of dentinogenic ghost cell tumour in the posterior maxillary bone of a teenager is reported, and the retrospective analysis is made to improve the understanding. References | Related Articles | Metrics

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Pedicled Palatal Periosteal Flap for the Closure of Oroantral Fistula: A Case Report CHAI Maosheng, CAI Yu 2022, 38(10): 997-999.  DOI: 10.13701/j.cnki.kqyxyj.2022.10.021 Abstract ( 213 )   PDF (1458KB) ( 243 )   The oroantral communications are usually happened after the removing of maxillary premolars and molars. Though most of these communications can heal with proper treatments, there are still a few cases with poor healing, and consequently the forming of oroantral fistula which is refractory and would cause intractable infection of maxillary sinus. This paper reported a case of oroantral fistula which was successfully repaired with palatal periosteal flap. The successful resolution of inflammation and the correct flap design might be the critical factors for the treatment. References | Related Articles | Metrics