Proliferation and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cell in Chitosan Thermosensitive Hydrogel.

Abstract

Abstract: Objective: To investigate the proliferation and osteogenic differentiation of human umbilical cord mesenchymal stem cells in chitosan thermosensitive hydrogel. Methods: Human umbilical cord mesenchymal stem cells were isolated and cultured in vitro. The chitosan thermosensitive hydrogel was visualized by scanning electron microscopy. Then the cells were cultured in chitosan thermosensitive hydrogel extracts and given osteogenic induction. The proliferation was measured by MTT assay. Alizarin red staining at 21d was performed to assess the osteogenic differentiation potential of hUCMSCs. Results: In chitosan thermosensitive gel, the proliferation of hUCMSCs was similar with those in common medium, and there was no significant difference. Alizarin red staining was positive with formation of calcium nodules after osteogenic induction (21d). Conclusion: HUCMSCs could proliferate and have osteogenic differentiation potential in chitosan thermosensitive hydrogel.

Key words: Human umbilical cord mesenchymal stem cell , Chitosan thermosensitive hydrogel , Proliferation, Osteogenic differentiation

CLC Number:

R782.2

WANG Yi-bo, LI Yun-peng, HOU Rui, et al. Proliferation and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cell in Chitosan Thermosensitive Hydrogel.[J]. Journal of Oral Science Research, 2015, 31(1): 38-40.

References

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