Effect of Glucose Level on Osteogenic Differentiation of Mouse Embryonic Stem Cells in 3D Culture System

Abstract

Abstract: Objective: To explore the possibility of gelatin sponge as a scaffold of mouse embryonic stem cells (ESCs) in vitro cultivation and to investigate the effect of glucose level on osteogenic differentiation of mouse ESCs. Methods: Mouse ESCs were cultured in vitro, and the dispersed cells were implanted into absorbable gelatin sponge for osteogenic induction. Three groups were set in the study: high-glucose-level group, low-glucose-level group and spontaneous differentiation group. After 7 and 14 days of osteogenic differentiation, the matrix mineralization was detected by alizarin red S staining. Results: After 7 and 14 days of osteogenic induction, low-glucose-level group had a higher percentage of alizarin red S positive cells, compared with high-glucose-level group and spontaneous differentiation group(P<0.05). Conclusion: Gelatin sponge can be used as a scaffold for culturing mouse ESCs in vitro study. High glucose level inhibits osteogenetic differentiation of mouse ESCs in the 3D culture system.

Key words: Embryonic stem cells, Gelatin sponge, Concentration of glucose, Osteogenic differentiation

CLC Number:

R782.1

GAO Yu-wei, ZHANG Yu, XING Jian-yu, et al. Effect of Glucose Level on Osteogenic Differentiation of Mouse Embryonic Stem Cells in 3D Culture System[J]. Journal of Oral Science Research, 2015, 31(8): 759-.

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