Abstract: Objective: To investigate the effect of room temperature curing polymethylmethacrylate (PMMA) material with the addition of 2% nano-silver base inorganic antibacterial agent on reproductive system of male mice. Methods: The male kunming mice were randomly divided into four groups, and were killed after animal models were established. The body weight growth rate and the testis tissue coefficient were calculated, and the pathological changes of testis were observed. The rate of sperm motility was detected. The rate of count and abnormal rate were also calculated. The spermatogonium sister chromatid exchange (SCE) test and primary spermatocyte chromosome aberration test were undertaken respectively to detect the effect of room curing polymethylmethacrylate (PMMA) material added 2% nano-silver base inorganic antibacterial agent on the chromosome of spermatogonium and primary spermatocyte. Results: There was no significant effect of room temperature curing PMMA added 2% nano-silver base inorganic antibacterial agent on the growth rate of body weight and testicular organ coefficient, sperm count, sperm motility and abnormal sperm rate. Testis pathological sections between the experiment groups and negative group were not obvious different. The significant effect of room temperature curing PMMA added 2% nano-silver base inorganic antibacterial agent on the chromosome of primary spermatocyte was not found. There were no significant differences in SCE frequency between experimental groups and the negative group. Conclusion: Room temperature curing PMMA material added 2% nano-silver base inorganic antibacterial agent has no significant effect on reproductive system of male mice.

Key words: Nano-silver base inorganic antibacterial agent, Polymethylmethacrylate, Chromosome aberration, Sister chromatid exchange