S1P/S1PR1 Regulates Chemotaxis of Osteoclast Precursors under Compressive Stress.

doi:10.13701/j.cnki.kqyxyj.2016.03.002

Abstract

Abstract: Objective: To investigate the role of Sphingosine-1-phosphate/Sphingosine-1-phosphate receptor 1 (S1P/S1PR1) axis in regulating migration of osteoclast precursors under compressive stress. Methods: Compressed model was built in vitro. The mRNA and protein expression levels of SPHK1 and S1PR1 in Raw 264.7 cell lines under compressive stress were investigated with real-time PCR, Western-blotting and immunofluoresecence. The regulatory role of S1P/S1PR1 signaling on chemotaxis of Raw 264.7 cell lines under compressive stress was explored. Results: Under compressive force of 0.5, 1.0 and 2.0 g/cm², SPHK1 and S1PR1 expressions and chemotaxis of Raw 264.7 cell lines were downregulated. And S1P/S1PR1 signaling functional antagonist FTY720 increased the chemotaxis of Raw 264.7 cells. Conclusion: S1P/S1PR1 axis regulates migration of osteoclast precursors under compressive stress.

Key words: S1P/S1PR1 axis, Compressive stress , Osteoclast precursors , Migration

CLC Number:

R783.5

FANG Jie, YE Xi, LI Yu-hong, HUANG Sheng-fu. S1P/S1PR1 Regulates Chemotaxis of Osteoclast Precursors under Compressive Stress.[J]. Journal of Oral Science Research, 2016, 32(3): 216-219.

References

[1] Huang H, Williams RC, Kyrkanides S. Accelerated orthodontic tooth movement: molecular mechanisms [J]. Am J Orthod Dentofacial Orthop, 2014, 146(5)∶620-632
[2] Kanzaki H, Shinohara F, Itohiya-Kasuya K, et al. Nrf2 activation attenuates both orthodontic tooth movement and relapse [J]. J Dent Res, 2015, 94(6)∶787-794
[3] Tsay TP, Chen MH, Oyen OJ. Osteoclast activation and recruitment after application of orthodontic force [J]. Am J Orthod Dentofacial Orthop, 1999, 115(3)∶323-330
[4] 杨健,谭颖徽,裘松波.核因子-κB受体活化因子配体在大鼠正畸牙压力侧骨改建中的作用[J].口腔医学研究,2013,20(3)∶259-262
[5] Faccio R, Teitelbaum SL, Fujikawa K,et al. Vav3 regulates osteoclast function and bone mass [J]. Nat Med, 2005, 11(3)∶284-290
[6] Prager B, Spampinato SF, Ransohoff RM. Sphingosine 1-phosphate signaling at the blood-brain barrier [J]. Trends in molecular medicine, 2015, 21(6)∶354-363
[7] Emanuel BR, Toshihiro I, Aki K, et al. Effect of PGE2 induced by compressive and tensile stresses on cementoblast differentiation in vitro [J]. Archives of oral biology, 2011, 56∶238-246
[8] Ishii T, Shimazu Y, Nishiyama I, et al. The role of sphingosine 1-phosphate in migration of osteoclast precursors; an application of intravital two-photon microscopy [J]. Mol Cells, 2011, 31(5)∶399-403
[9] Ryu J, Kim HJ, Chang EJ, et al. Sphingosine 1-phosphate as a regulator of osteoclast differentiation and osteoclast-osteoblast coupling [J]. EMBO J, 2006, 25(24)∶5840-51
[10] Quint P, Ruan M, Pederson L, et al. Sphingosine 1-phosphate (S1P) receptors 1 and 2 coordinately induce mesenchymal cell migration through S1P activation of complementary kinase pathways [J]. J Biol Chem, 2013, 288(8)∶5398-406
[11] Ishii M, Kikuta J, Shimazu Y, et al. Chemorepulsion by blood S1P regulates osteoclast precursor mobilization and bone remodeling in vivo [J]. J Exp Med, 2010, 207(13)∶2793-2798
[12] Ishii M, Egen JG, Klauschen F, et al. Sphingosine-1-phosphate mobilizes osteoclast precursors and regulates bone homeostasis [J]. Nature, 2009, 458(7237)∶524-528

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