Influence of Nuclear Localization Sequence and C-Terminal Deletion of Parathyroid Hormone Relative Peptide on Mandible Development in Mice

doi:10.13701/j.cnki.kqyxyj.2017.10.006

Abstract

Abstract: Objective: To study the influence of nuclear localization sequence (NLS) and C-terminal deletion of parathyroid hormone relative peptide (PTHrP) on the mandible development in mice. Methods: Computed tomography (CT) scanning, hematein eosin (HE) staining, total collagen staining, alkaline phosphatase (ALP) staining, tartrate-resistant acid phosphatase (TRAP) staining, immunohistochemical staining, and western-blot were used to compare the developmental difference of the first molar in mandible between PTHrP knock mice (PTHrP KI group, n=10) and wild type mice (WT group). Results: Osteoblast density, mandible relative bone mass, ALP positive area, and collagen-I positive area of WT group were markedly higher than PTHrP KI group (P<0.05). However, expressions of mandible p16, p21, and p27 proteins of PTHrP KI group were higher than that of the WT group, while Ki-67 protein was lower than that of the WT group, and the differences were statistical significant (P<0.05). Conclusion: NLS and C-terminal deletion of PTHrP induce the decrease of osteoblasts and inhibit the synthesis and mineralization of extracellular matrix, thus leading to the mandible development disorder in mice.

Key words: Parathyroid hormone, related peptide, Nuclear localization sequence, C-terminal, Mandible, Development

CLC Number:

R782

LIU HONG, YAN Cheng-dong , JIANG Shang-fei. Influence of Nuclear Localization Sequence and C-Terminal Deletion of Parathyroid Hormone Relative Peptide on Mandible Development in Mice[J]. Journal of Oral Science Research, 2017, 33(10): 1044-1047.

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