Modification of Rat Model with Peri-implantitis

doi:10.13701/j.cnki.kqyxyj.2021.04.009

Abstract

Abstract: Objective: To simplify the steps and shorten the time of reconstruction of rat peri-implantitis model. Methods: The maxillary first molars of 16 rats were extracted, and titanium implants were implanted one month later. After 4 weeks, silk thread ligation was used to induce inflammation and the rats were fed with sugar water. Two weeks later, the rats were sacrificed, the maxilla was taken X-rays for imaging analysis, bone tissue slices were made, and the gingival tissue around the implant was extracted for immunohistochemical analysis. Results: Two weeks after ligation, peri-implantitis was successfully induced. There was not only soft tissue inflammation around the implant, but also bone tissue resorption at various degrees. Conclusion: The model of rat peri-implantitis has been successfully constructed, with convenience and short modeling cycle.

Key words: peri-implantitis, rat, titanium implant, osseointegration

LI Xingjia, CHEN Qixin, YUAN Changyong, WANG Penglai. Modification of Rat Model with Peri-implantitis[J]. Journal of Oral Science Research, 2021, 37(4): 314-318.

References

[1] Schwarz F, Derks J, Monje A, et al. Peri-implantitis [J]. J Periodontol, 2018, 89 Suppl 1: S267-S290.
[2] Berglundh T, Jepsen S, Stadlinger B, et al. Peri-implantitis and its prevention [J]. Clin Oral Implants Res, 2019, 30(2): 150-155.
[3] Berglundh T, Armitage G, Araujo MG, et al. Peri-implant diseases and conditions: Consensus report of workgroup 4 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions [J]. J Periodontol, 2018, 89 Suppl 1: S313-S318.
[4] Dreyer H, Grischke J, Tiede C, et al. Epidemiology and risk factors of peri-implantitis: A systematic review [J]. J Periodontal Res, 2018, 53(5): 657-681.
[5] Roccuzzo M, Layton DM, Roccuzzo A, et al. Clinical outcomes of peri-implantitis treatment and supportive care: A systematic review [J]. Clin Oral Implants Res, 2018, 29 Suppl 16: 331-350.
[6] Khoury F, Keeve PL, Ramanauskaite A, et al. Surgical treatment of peri-implantitis -consensus report of working group 4 [J]. Int Dent J, 2019, 69 Suppl 2: 18-22.
[7] Takamori Y, Atsuta I, Nakamura H, et al. Histopathological comparison of the onset of peri-implantitis and periodontitis in rats [J]. Clin Oral Implants Res, 2017, 28(2): 163-170.
[8] 孔晨,李永丽,李思佳,等.牙周炎动物模型的研究进展[J].医学综述,2019,25(17): 3344-3349.
[9] Ting M, Craig J, Balkin BE, et al. Peri-implantitis: a comprehensive overview of systematic reviews [J]. J Oral Implantol, 2018, 44(3): 225-247.
[10] Caton JG, Armitage G, Berglundh T, et al. A new classification scheme for periodontal and peri-implant diseases and conditions-Introduction and key changes from the 1999 classification [J]. J Periodontol, 2018, 89 Suppl 1: S1-S8.
[11] Chevalier M, Ranque S, Precheur I. Oral fungal-bacterial biofilm models in vitro: a review [J]. Med Mycol, 2018, 56(6): 653-667.
[12] Ramanauskaite A, Becker K, Schwarz F. Clinical characteristics of peri-implant mucositis and peri-implantitis [J]. Clin Oral Implants Res, 2018, 29(6): 551-556.
[13] 李娜,林崇翔,丁熙,等.大鼠微螺钉种植体周围炎外周血中Foxp3⁺调节性T细胞的水平变化[J].口腔医学研究, 2018, 34(7): 726-729.
[14] 朱白雪,高晓蔚,戴晓玮.种植体周围炎动物模型的研究进展[J].口腔医学研究,2018,38(8): 747-751.
[15] 吴佩佩,姑则丽努尔·阿布都克力木, 朱亚玲,等.维药买朱尼对犬种植体周围炎骨改建的实验研究[J].口腔医学研究, 2018, 34(9): 974-978.
[16] Hiyari S, Wong RL, Yaghsezian A, et al. Ligature-induced peri-implantitis and periodontitis in mice [J]. J Clin Periodontol, 2018, 45(1): 89-99.
[17] Sun J, Eberhard J, Glage S, et al. Development of a peri-implantitis model in the rat [J]. Clin Oral Implants Res, 2020, 31(3): 203-214.
[18] Elemek E, Agrali OB, Kuru B, et al. Peri-implantitis and severity level [J]. Eur J Dent, 2020, 14(1): 24-30.
[19] Romanos GE, Delgado-Ruiz R, Sculean A. Concepts for prevention of complications in implant therapy [J]. Periodontol 2000, 2019, 81(1): 7-17.
[20] Heitz-Mayfield LJA, Salvi GE, Mombelli A, et al. Supportive peri-implant therapy following anti-infective surgical peri-implantitis treatment: 5-year survival and success [J]. Clin Oral Implants Res, 2018, 29(1): 1-6.
[21] Heo S, Kim HJ, Joo JY, et al. Simplified nonsurgical treatment of peri-implantitis using chlorhexidine and minocycline hydrochloride [J]. J Periodontal Implant Sci, 2018, 48(5): 326-333.
[22] Isler SC, Soysal F, Ceyhanli T, et al. Regenerative surgical treatment of peri-implantitis using either a collagen membrane or concentrated growth factor: A 12-month randomized clinical trial [J]. Clin Implant Dent Relat Res, 2018, 20(5): 703-712.
[23] Mercado F, Hamlet S, Ivanovski S. Regenerative surgical therapy for peri-implantitis using deproteinized bovine bone mineral with 10% collagen, enamel matrix derivative and Doxycycline-A prospective 3-year cohort study [J]. Clin Oral Implants Res, 2018, 29(6): 583-591.
[24] Kubasiewicz-Ross P, Hadzik J, Gedrange T, et al. Antimicrobial efficacy of different decontamination methods as tested on dental implants with various types of surfaces [J]. Med Sci Monit, 2020, 26: e920513.
[25] Chackartchi T, Romanos GE, Sculean A. Soft tissue-related complications and management around dental implants [J]. Periodontol 2000, 2019, 81(1): 124-138.
[26] De Oliveira P, Bonfante E, Bergamo E, et al. Obesity/metabolic syndrome and diabetes mellitus on peri-implantitis [J]. Trends Endocrinol Metab, 2020, 31(8): 596-610.
[27] 丁修明.炎症细胞因子IL-6、TNF-α和MMP-8在口腔种植体周围炎患者中的变化[J].基因组学与应用生物学,2019,38(11): 5335-5339.
[28] 冷春涛,李婷,高晓蔚.4种白细胞介素在种植体周围炎分期模型中的表达分析[J].上海口腔医学,2020,29(2): 162-167.
[29] 刘晨, 毕良佳. 肿瘤坏死因子-α在慢性牙周炎中的研究进展[J].中华老年口腔医学杂志,2019,17(5): 308-313.
[30] Nguyen Vo TN, Hao J, Chou J, et al. Ligature induced peri-implantitis: tissue destruction and inflammatory progression in a murine model [J]. Clin Oral Implants Res, 2017, 28(2): 129-136.
[31] Ghassib I, Chen Z, Zhu J, et al. Use of IL-1 beta, IL-6, TNF-alpha, and MMP-8 biomarkers to distinguish peri-implant diseases: A systematic review and meta-analysis [J]. Clin Implant Dent Relat Res, 2019, 21(1): 190-207.
[32] Gomes AM, Douglas-De-Oliveira DW, Ferreira SD, et al. Periodontal disease, peri-implant disease and levels of salivary biomarkers IL-1beta, IL-10, RANK, OPG, MMP-2, TGF-beta and TNF-alpha: follow-up over 5 years [J]. J Appl Oral Sci, 2019, 27: e20180316.
[33] He K, Jian F, He T, et al. Analysis of the association of TNF-alpha, IL-1A, and IL-1B polymorphisms with peri-implantitis in a Chinese non-smoking population [J]. Clin Oral Investig, 2020, 24(2): 693-699.
[34] 贺平,冯瑜,李冀寅,等.潜入式种植修复10年回顾性研究[J].口腔医学研究,2019,35(2): 168-171.
[35] Galofre M, Palao D, Vicario M, et al. Clinical and microbiological evaluation of the effect of Lactobacillus reuteri in the treatment of mucositis and peri-implantitis: A triple-blind randomized clinical trial [J]. J Periodontal Res, 2018, 53(3): 378-390.
[36] Hirabayashi M, Takizawa A, Hochi S. Embryonic stem cells and gene manipulation in rat [J]. Methods Mol Biol, 2019, 2018: 115-130.
[37] Liang D, Wang KJ, Tang ZQ, et al. Effects of nicotine on the metabolism and gene expression profile of SpragueDawley rat primary osteoblasts [J]. Mol Med Rep, 2018, 17(6): 8269-8281.
[38] Lu J, Chen A, Ma X, et al. Generation and characterization of cytochrome p450 2J3/10 CRISPR/Cas9 knockout rat model [J]. Drug Metab Dispos, 2020, 48(11): 1129-1136.