Repair of Rabbits Critical Mandibular Defects with BMSCs/NHAC Complex

doi:10.13701/j.cnki.kqyxyj.2018.01.006

Abstract

Abstract: Objective: To investigate the effect of BMSCs/NHAC complex on repair of rabbit's critical mandibular defects. Methods: MTS experiments were used to detect cell proliferation. ALP activity was measured by alkaline phosphatase activity assay. The osteogenic gene was detected by qRT-PCR. The complex was implanted into the mandibular critical defect of rabbit. Goldner trichrome staining test and micro-CT were used to evaluate bone regeneration. Results: OD490 values were significantly higher in the co-culture group than that in the normal group (P<0.05). ALP activity in the co-culture-induced group was significantly higher than that in the normal induction group, as well as the expression levels of OCN, Runx2, and Osterix mRNA (P<0.05). Compared with the black group, the bone volume and bone density of NHAC group and BMSCs/NHAC group were significantly increased (P<0.05). Conclusion: BMSCs/NHAC complexes can promote the bone regeneration in the mandibular critical defect of rabbit.

Key words: Bone marrow mesenchymal stem cells, Nanocrystalline collagen bone material, Tissue engineering

CLC Number:

LI Dong-mei, LIU Xin-hui, LI Qing-xing.. Repair of Rabbits Critical Mandibular Defects with BMSCs/NHAC Complex[J]. Journal of Oral Science Research, 2018, 34(1): 22-26.

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