Effects of Estrogen Receptor on Osteogenic Differentiation of Periodontal Ligament Stem Cells in Ovariectomized Rats.

doi:10.13701/j.cnki.kqyxyj.2018.04.025

Abstract

Abstract: Objective: To investigate the effect of estrogen receptor on osteogenic differentiation of periodontal ligament stem cells (PDLSCs) by constructing a low estrogen microenvironment. Methods: Sixteen female SD rats were randomly divided into ovariectomized group and sham operation group, who were given surgical treatment accordingly. Real-time quantitative PCR was used to measure the rat estrogen receptor and osteogenic index. Results: The weight of OVX group was significantly higher than that in the sham operation group, and estradiol and bone density were significantly lower than those of sham operation group (P<0.05). The ALP activities of OVX group after 3, 5, 7, 9, and 11 d were higher than those of sham operation group (P<0.05), however, no difference at 1d and 13 d. The levels of ERα mRNA of OVX group after 5, 9, 11, and 13 d were significantly lower than those of sham operation group, and the levels of ERβ mRNA of OVX group after 5, 7, 11, and 13 d were significantly lower than those in sham operation group. The levels of ALP mRNA, BSP mRNA of OVX group after 5, 7, 9, 11, and 13 d were significantly lower than those in sham operation group (P<0.05). Conclusion: The osteogenic ability of PDLSCs in ovariectomized rat is correspondingly reduced, and the estrogen receptor plays an important role on osteogenic differentiation ability of PDLSCs.

Key words: Estrogen receptor, Periodontal ligament stem cells, Osteogenic differentiation ability

SHEN Lan-hua, ZHANG Rui, MENG Ling-na. Effects of Estrogen Receptor on Osteogenic Differentiation of Periodontal Ligament Stem Cells in Ovariectomized Rats.[J]. Journal of Oral Science Research, 2018, 34(4): 448-451.

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