Effects of PTH-FC on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

doi:10.13701/j.cnki.kqyxyj.2018.06.010

Abstract

Abstract: Objective: To evaluate the effects of PTH-FC on osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), and to screen out the optimal time of BMSCs induced by PTH-FC. Methods: BMSCs from rats were isolated and cultured in vitro using the whole bone marrow adherence method and identified. BMSCs was induced with PTH-FC (10^-9mol/L) osteogenic inducer for 6h (intermittent administration) and 48h (continuous administration). The best proliferation promoting and osteogenic differentiation promoting effect of PTH-FC on BMSCs was evaluated by means of MTT, ALP, and alizarin red staining. Results: ALP and alizarin red staining in the osteogenic culture medium of PTH-FC (10^-9mol/L) which acted on BMSCs for 6h were significantly higher than those of other groups (P<0.05), however, the cell proliferation result was not significant different between the experimental groups. Conclusion: The combination of PTH-FC, which is used as a bone induced growth factor, with BMSCs to repair bone tissue defects is feasible.

Key words: Parathyroid hormone-FC, Bone marrow mesenchymal stem cells, Osteogenic differentiation

LIU Yan-yun, XU Ning, LIU Yu-hui, LIU Jia-tao, ZHU Yi-dan, HE Miao-miao, YU Zhan-hai. Effects of PTH-FC on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells[J]. Journal of Oral Science Research, 2018, 34(6): 614-618.

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