Effects of D-Histidine on Streptococcus Mutans Biofilm.

doi:10.13701/j.cnki.kqyxyj.2019.02.018

Abstract

Abstract: Objective s: To evaluate the effects of D-Histidine on the Streptococcus mutans biofilm in vitro. Methods: Antimicrobial activity testing of D-Histidine was evaluated by determining the growth curve. Crystal violet biofilm biomass assay and polysaccharide measurement were performed to evaluate the potential activity of D-Histidine effectively inhibiting biofilm formation. SEM was used to observe the morphology and the structure of biofilm. Results: Growth curve showed that D-Histidine at concentration of 20% interfered with the viability of Streptococcus mutans in a dose-dependent manner. CV and EPS assays showed that D-Histidine at concentration of 40% effectively inhibited Streptococcus mutans biofilm formation and the polysaccharide level of Streptococcus mutans biofilm was greatly reduced. SEM results displayed that D-Histidine effectively inhibited Streptococcus mutans bioflim formation at concentration of 40% and bacterial aggregation amount declined with increasing concentrations of D-Histidine. Conclusion: D-Histidine effectively inhibits Streptococcus mutan biofilm formation in a dose-dependent manner and D-Histidine interferes with the viability of Streptococcus mutans.

Key words: Biofilm, Streptococcus mutans, D-Histidine

QUAN Xu, XU Tong-tong, ZHANG Hui-yan, WANG He-ling, WANG Chun-yang, CUI Yun-xia, FU Qi-yue, MENG Wei-yan.. Effects of D-Histidine on Streptococcus Mutans Biofilm.[J]. Journal of Oral Science Research, 2019, 35(2): 176-179.

References

[1] Dewhirst FE, Chen T, Izard J, et al. The human oral microbiome [J]. J Bacteriol, 2010, 192(19): 5002-5017.
[2] Wade WG. The oral microbiome in health and disease [J]. Pharmacol Res, 2013, 69(1): 137-143.
[3] 史彦,李成杰,张明正,等.龋齿及牙周病患者与健康人群口腔微生态的比较研[J].口腔医学研究,2016,32(12): 1265-1268.
[4] Seminario A, Broukal Z, Ivancaková R. Mutans streptococci and the development of dental plaque [J]. Prague Med Rep, 2005, 106(4): 349-358.
[5] Tamura S, Yonezama H, Motegi M, et al. Inhibiting effects of Streptococcus salivarius on competence-stimulating peptide-dependent biofilm formation by Streptococcus mutans [J]. Oral Microbiol Immunol, 2009, 24(2): 152-161.
[6] Krysciak W, Jurczak A, Koscielniak D, et al. The virulence of Streptococcus mutans and the ability to form biofilms [J]. Eur J Clin Microbiol Infect Dis, 2014, 33(4): 499-515.
[7] Marsh PD. Microbiology of dental plaque biofilms and their role in oral health and caries [J]. Dent Clin North Am, 2010, 54(3): 441-454.
[8] Pensyan A, Gillngs M, Paulsen IT. Antibiotic discovery: combatting bacterial resistance in cells and in biofilm communities [J]. Molecules, 2015, 20(4): 5286-5298.
[9] Qi H, Li B, Wang H, et al. Effects of d-valine on periodontal or peri-implant pathogens: Porphyromonas gingivalis biofilm [J]. J Periodontol, 2018, 89(3): 303-314.
[10] Kolodkin-Gal I, Romero D, Cao S, et al. D-amino acids trigger biofilm disassembly [J]. Science, 2010, 328(5978): 627-629.
[11] Leiman SA, May JM, Lebar MD, et al. D-amino acids indirectly inhibit biofilm formation in Bacillus subtilis by interfering with protein synthesis [J]. J Bacteriol, 2013, 195(23): 5391-5395.
[12] She P, Chen L, Liu H, et al. The effects of D-Tyrosine combined with amikacin on the biofilms of Pseudomonas aeruginosa [J]. Microb Pathog, 2015, 86:38-44.
[13] Pitts B, Hamilton MA, Zelver N, et al. A microtiter-plate screening method for biofilm disinfection and removal [J]. J Microbiol Methods, 2003, 54(2): 269-276.
[14] Wang L, Melo MA, Weir MD, et al. Novel bioactive nanocomposite for Class-Ⅴ restorations to inhibit periodontitis-related pathogens [J]. Dent Mater, 2016, 32(12): e351-e361.
[15] Yang Y, Sreenivasan PK, Subramanyam R, et al. Multiparameter assessments to determine the effects of sugars and antimicrobials on a polymicrobial oral biofilm [J]. Appl Environ Microbiol, 2006, 72(10): 6734-6742.
[16] Takahashi N, Nyvad B. The role of bacteria in the caries process: ecological perspectives [J]. J Dent Res, 2011, 90(3): 294-303.
[17] ten Cate JM, Cummins D. Fluoride toothpaste containing 1.5% arginine and insoluble calcium as a new standard of care in caries prevention [J]. J Clin Dent, 2013, 24(3): 79-87.
[18] Lam H, Oh DC, Cava F, et al. D-amino acids govern stationary phase cell wall remodeling in bacteria [J]. Science, 2009, 325(5947): 1552-1555.
[19] Flemming HC, Neu TR, Wozniak DJ. The EPS matrix: the "house of biofilm cells" [J]. J Bacteriol, 2007, 189(22): 7945-7947.
[20] Cava F, Lam H, De Pedro MA, et al. Emerging knowledge of regulatory roles of D-amino acids in bacteria [J]. Cell Mol Life Sci, 2011, 68(5): 817-831.
[21] Jia R, Li Y, Al-Mahamedh HH, et al. Enhanced biocide treatments with D-amino acid mixtures against a biofilm consortium from a water cooling tower [J]. Front Microbiol, 2017, 8:1538.