Effect of Down-regulation of Mucin-1 on Growth of Xenografts of Human Tongue Squamous Cell Carcinoma Tca-8113 in Nude Mice

doi:10.13701/j.cnki.kqyxyj.2019.08.013

Abstract

Abstract: Objective: To investigate the effect of down-regulation of mucin-1 (MUC1) expression on the growth of xenografts of human tongue squamous cell carcinoma Tca-8113 in nude mice. Methods: Tca-8113 cells were infected with MUC1-short hairpin RNA (shRNA) lentivirus. Quantitative real-time PCR (qPT-PCR) and western blot were used to detect the expression of MUC1 in infected Tca-8113 cells. The squamous cell carcinoma of tongue xenografts in nude mice were constructed by subcutaneous injection of Tca-8113 cells infected with MUC1-shRNA lentivirus. The volume of tumors was observed and the growth curve was drawn. Hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining were used to observe the pathological changes and apoptosis of xenografts. The expression of MUC1, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X (Bax), Cleaved-cysteine aspartic acid specific protease-3 (Cleaved-caspase-3), and proliferating cell nuclear antigen (PCNA) in tumors were detected by western blot. Results: After infected with MUC1-shRNA lentivirus, the mRNA and protein expression of MUC1 in Tca-8113 cells decreased significantly (P<0.05). Compared with the control group and negative control group (MUC1-shCON), the volume of tumors in the MUC1-shRNA group was significantly reduced (P<0.05), the apoptosis of transplanted tumor tissue was serious, and the expressions of MUC1, Bcl-2 and PCNA were significantly decreased (P<0.05), while the expressions of Bax and cleaves-caspase-3 protein were increased (P< 0.05). Conclusion: The down-regulation of MUC1 can significantly inhibit the growth of tumor xenograft of human tongue squamous cell carcinoma Tca-8113 in nude mice.

Key words: Mucin-1, tongue squamous cell carcinoma, xenografts, apoptosis

DENG Xuan, YANG Fang, TANG Xiqing. Effect of Down-regulation of Mucin-1 on Growth of Xenografts of Human Tongue Squamous Cell Carcinoma Tca-8113 in Nude Mice[J]. Journal of Oral Science Research, 2019, 35(8): 776-780.

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