Nell-1 Regulates Osteogenic Differentiation of Human Maxillary Sinus Membrane-derived Mesenchymal Stem Cells through Wnt/β-catenin Pathway

doi:10.13701/j.cnki.kqyxyj.2023.03.009

Abstract

Abstract: Objective: To investigate the effect of Nell-1 protein on osteogenic differentiation of human maxillary sinus membrane-derived mesenchymal stem cells (MSMSCs). Methods: The MSMSCs were induced by Nell-1 protein (10,100,1000 ng/mL), and cell activity changes were detected using CCK-8 assay and cell live-dead staining. The changes in osteogenic ability were detected by alkaline phosphatase activity. mRNA expression of osteogenic-related factors was detected by quantitative real-time fluorescence PCR (qRT-PCR). Immunofluorescence staining and western blot were used to detect osteogenic protein expression. Results: Nell-1 protein had a promote effect on proliferative of MSMSCs. Alkaline phosphatase activity assay showed that the osteogenic effect of each experimental group was better than that of the control group. qRT-PCR, immunofluorescence staining, and western blot showed that 100 ng/mL of Nell-1 significantly promoted the mRNAs and protein expression of β-catenin, Runx-2, OCN, and others (P<0.01). Conclusion: Nell-1 protein can improve the osteogenic differentiation ability of MSMSCs in vitro by upregulating the expression of Wnt/β-catenin signaling pathway.

Key words: mesenchymal stem cells, bone regeneration, maxillary sinus, Nell-1

XU Jing, LV Huixin, WANG Yihan, BAO Xin, ZHANG Yi, ZHOU Yanmin. Nell-1 Regulates Osteogenic Differentiation of Human Maxillary Sinus Membrane-derived Mesenchymal Stem Cells through Wnt/β-catenin Pathway[J]. Journal of Oral Science Research, 2023, 39(3): 227-235.

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