Role of Phosphodiesterase 4D in the Pathogenesis of Oral Squamous Cell Carcinoma.

doi:10.13701/j.cnki.kqyxyj.2017.06.021

Abstract

Abstract: Objective: To investigate the role of phosphodiesterase 4D in the pathogenesis of oral squamous cell carcinoma (OSCC). Methods: Fifty-three OSCC patients and 5 OSCC cell lines were collected, 55 healthy adults and 2 normal oral epithelial cells were used as the control. The mRNA levels of PDE4D in these samples, the proliferation of SSC25, the migration abilities, and the cell apoptosis were examined by quantitative RT-PCR, CCK-8, wounding healing assay, and flow cytometry as well as western blot, respectively. Results: The levels of PDE4D mRNA in OSCC tissue and cell lines were significant higher than that in normal control group (P<0.01); the levels of PDE4D mRNA in OSCC tissue were positively correlated with the degree of OSCC differentiation (P<0.05); the abilities of cell proliferation and migration were significantly inhibited by Rolipram; Rolipram induced a significant apoptotic changes on SCC25 cells (P<0.05). Conclusion: PDE4D plays an important role in the OSCC pathogenesis.

Key words: Oral squamous cell carcinoma , Phosphodiesterase, 4D, Proliferation, Migration , Apoptosis

CLC Number:

R739.8

XIA Dong-jing, HUANG Ying-ying, LI Hai-peng, RUI Hang.. Role of Phosphodiesterase 4D in the Pathogenesis of Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2017, 33(6): 663-667.

References

[1] Radhika T, Jeddy N, Nithya S, et al. Salivary biomarkers in oral squamous cell carcinoma-An insight [J]. J Oral BiolCraniofac Res, 2016, 6(Suppl 1)∶S51-S54
[2] Malik UU,Zarina S, Pennington SR.Oral squamous cell carcinoma: Key clinical questions, biomarker discovery, and the role of proteomics [J]. Arch Oral Biol, 2016, 63(1)∶53-65
[3] Jrgensen C, Yasmeen S, Iversen HK, et al. Phosphodiesterase4D (PDE4D)--A risk factor for atrial fibrillation and stroke [J]. J NeurolSci, 2015, 359(1-2)∶266-274
[4] Gurney ME, D'Amato EC, Burgin AB. Phosphodiesterase-4 (PDE4) molecular pharmacology and Alzheimer's disease [J]. Neurotherapeutics, 2015, 12(1)∶49-56
[5] Michot C, Le Goff C, Goldenberg A, et al. Exome sequencing identifies PDE4D mutations as another cause of acrodysostosis [J]. Am J Hum Genet, 2012, 90(4)∶740-745
[6] Bttcher R, Henderson DJ, Dulla K et al. Human phosphodiesterase 4D7 (PDE4D7) expression is increased in TMPRSS2-ERG-positive primary prostate cancer and independently adds to a reduced risk of post-surgical disease progression [J]. Br J Cancer, 2015, 113(10)∶1502-1511
[7] Cao B, Wang K, Liao JM, et al. Inactivation of oncogenic cAMP-specific phosphodiesterase 4D by miR-139-5p in response to p53 activation [J]. Elife, 2016,5,pii∶e15978
[8] 俞力夫,代晓明,李逸松.mRNA差异表达与口腔鳞状细胞癌相关研究进展[J].口腔医学研究, 2016,32(1)∶100-103
[9] 李宠,朱杨,山本哲也,等.口腔鳞癌细胞分泌的胞外体对其生物学行为的影响[J].口腔医学研究, 2016,32(8)∶800-803
[10] Mika D, Conti M. PDE4D phosphorylation: A coincidence detector integrating multiple signaling pathways [J]. Cell Signal, 2016, 28(7)∶719-724
[11] Fujita M, Hines CS, Zoghbi SS, et al.Downregulation of brain phosphodiesterase type IV measured with 11C-(R)-rolipram positron emission tomography in major depressive disorder [J]. Biol Psychiatry, 2012, 72(7)∶548-554
[12] Porpodis K, Domvri K, Zarogoulidis P, et al. Roflumilast, a phosphodiesterase-4 inhibitor, induces phagocytic activity in Greek COPD patients [J]. Int J Chron Obstruct Pulmon Dis, 2015, 10∶1123-1128
[13] Niknejad H, Yazdanpanah G, Ahmadiani A.Induction of apoptosis, stimulation of cell-cycle arrest and inhibition of angiogenesis make human amnion-derived cells promising sources for cell therapy of cancer [J]. Cell Tissue Res, 2016, 363(3)∶599-608

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