Effects of Long Non-coding RNA HOTAIR on Proliferation, Migration, and Invasion of Tongue Squamous Cell Carcinoma

doi:10.13701/j.cnki.kqyxyj.2018.06.013

Abstract

Abstract: Objective: To investigate the effect of HOTAIR on proliferation, migration, and invasion of tongue squamous cell carcinoma cell line. Methods: The expression of HOTAIR was knocked down by liposome mediated siRNA, and the meaningless sequence was set as the control group. QRT-PCR was used to detect the silencing effect. MTT assay was used to detect the proliferation of each group. The changes of invasion and migration ability of each group was detected by scratch test and Transwell test. Results: HOTAIR was successfully knocked down in the 2 cell lines, and the difference was statistically significant (P<0.001). In the MTT assay, compared with the control group, the absorbance values after 24h, 48h, 72h, and 96h in the experimental group were decreased (P<0.05). In the Transwell test, the membrane cell number of the experimental group was significantly less than that of the control group (P<0.001). The migration distance of the experimental group in the scratch group was less than that of the control group (P<0.001). Conclusion: HOTAIR promotes the proliferation, migration, and invasion of tongue squamous cells carcinoma (Tca-8113, TSCCA), which involved in the development of tongue cancer.

Key words: Tongue squamous cell carcinoma, Long-noncoding, RNA, HOTAIR, Proliferation, Migration, Invasion

DAI Meng-ying, ZHANG Yang, ZHANG Zhao-nan, SU Wei-peng, ZHANG Song-an, LIU Pan, ZHAO Hua-rong. Effects of Long Non-coding RNA HOTAIR on Proliferation, Migration, and Invasion of Tongue Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2018, 34(6): 627-631.

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