Diagnostic Values of Salivary MicroRNA-375 for OSCC and Its Effects on Biological Function of OSCC Cells

doi:10.13701/j.cnki.kqyxyj.2019.09.016

Abstract

Abstract: Objective: To explore the diagnostic value of salivary miR-375 for patients with oral squamous cell carcinomas (OSCC) and investigate the potential effects of miR-375 on biological characteristics of OSCC cells. Methods: Saliva samples from 20 OSCC patients and 20 healthy controls were collected. RT-PCR was applied to compare the relative expression of miR-375. SCC-4 cells were cultured and transfected with miR-375 mimics (miR-375 mimic group) via Lipofectamine 2000 liposome. HN-6 cells were cultured and transfected with miR-375 inhibitor (miR-375 inhibitor group) via Lipofectamine 2000 liposome. All cells without transfection were used as control group. The cell proliferation and cell apoptosis were measured by EDU and flow cytometry. The migration and invasion ability of the cells were detected by scratch test and Transwell test, respectively. Results: The expression of salivary miR-375 in OSCC patients was significantly lower than that in healthy control group (P<0.05). The low expression of miR-375 in SCC-4 cells, after miR-375 mimicking transfection, inhibited the cell proliferation, promoted the apoptosis, and decreased the cell migration and invasive ability. The highly expression of miR-375 in HN-6 cells, after miR-375 inhibitor transfection, promoted the cell proliferation, inhibited the apoptosis,and increased the cell migration and invasive ability. Conclusion: Our study indicates that miR-375 is down expression in the saliva of patients with OSCC and likes a tumor suppressor gene in OSCC, and it could be used as an indicator of diagnosis of OSCC in future.

Key words: microRNA-375, oral squamous cell carcinoma, proliferation, apoptosis, migration, invasion

XIE Long, SANG Lei, SONG Zhuoying, JIANG Yanjun. Diagnostic Values of Salivary MicroRNA-375 for OSCC and Its Effects on Biological Function of OSCC Cells[J]. Journal of Oral Science Research, 2019, 35(9): 883-888.

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