USP 12 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells under Cyclic Tension

doi:10.13701/j.cnki.kqyxyj.2021.06.011

Abstract

Abstract: Objective: To investigate the influence of ubiquitin-specific protease 12 (USP 12) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) under cyclic tension. Methods: Primary hPDLSCs were collected and the differentiation characteristics were detected by osteogenic, adipogenic, and chondrogenic differentiation experiments. The expression of USP 12 was detected by real-time quantitative PCR, Western blot, and immunofluorescence assay. The osteogenic differentiation of hPDLSCs was detected by ALP staining test. The expressions of ALP, OGT, and OPN were detected by real-time quantitative PCR. Results: The primary hPDLSCs had the potential of multi-directional differentiation. The expression of USP 12 increased at first and then decreased after cyclic stretch stimulation. The expression of ALP and OGT increased at first and then decreased, and the expression of OPN increased gradually. Conclusion: USP 12 may promote osteogenic differentiation of hPDLSCs under cyclic tension.

Key words: cyclic tension, human periodontal ligament stem cell, osteogenesis, ubiquitin-specific protease 12

ZOU Hao, CHANG Maolin, ZHANG Zhen, HAN Guangli. USP 12 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells under Cyclic Tension[J]. Journal of Oral Science Research, 2021, 37(6): 528-532.

References

[1] 邬扬绚,向黎,黄志强,等.釉基质蛋白衍生物调节牙龈卟啉单胞菌感染牙周膜干细胞成骨分化的作用及机制[J].口腔医学研究,2020,36(6):534-538.
[2] Yang Y, Wang BK, Chang ML, et al. Cyclic stretch enhances osteogenic differentiation of human periodontal ligament cells via YAP activation [J]. Biomed Res Int, 2018, 2018:2174824.
[3] Chang M, Lin H, Luo M, et al. Integrated miRNA and mRNA expression profiling of tension force-induced bone formation in periodontal ligament cells [J]. In Vitro Cell Dev Biol Anim, 2015, 51(8):797-807.
[4] Zong Z, Zhang Z, Wu L, et al. The functional deubiquitinating enzymes in control of innate antiviral immunity [J]. Adv Sci (Weinh), 202, 8(2):2002484.
[5] Chang M, Lin H, Fu H, et al. CREB activation affects mesenchymal stem cell migration and differentiation in periodontal tissues due to orthodontic force [J]. Int J Biochem Cell Biol, 2020, 129:105862.
[6] Yu H, Yu W, Liu Y, et al. Expression of HIF 1α in cycling stretch induced osteogenic differentiation of bone mesenchymal stem cells [J]. Mol Med Rep, 2019, 20(5):4489-4498.
[7] 孙超.葡萄糖诱导Sox9/Runx2 O-GlcNAc糖基化在软骨终板干细胞分化中的作用及机制研究[D].重庆:中国人民解放军陆军军医大学,2020.
[8] 张琳琳,安莹,陈发明,等.牙源性干细胞的研究进展[J].实用口腔医学杂志,2015,31(3):425-431.
[9] 李汝磊.ETS2对牙周膜干细胞成骨分化的影响[D].重庆:中国人民解放军陆军军医大学,2019.
[10] Liu S, de Boeck M, van Dam H, et al. Regulation of the TGF-β pathway by deubiquitinases in cancer [J]. Int J Biochem Cell Biol, 2016, 76:135-145.
[11] Guo YC, Wang MY, Zhang SW, et al. Ubiquitin-specific protease USP34 controls osteogenic differentiation and bone formation by regulating BMP2 signaling [J]. EMBO J, 2018, 37(20):e99398.
[12] Williams S, Maecker H, French D, et al. USP1 deubiquitinates ID proteins to preserve a mesenchymal stem cell program in osteosarcoma [J]. Cell, 2011, 146(6):918-930.
[13] Zhou F, Li F, Fang P, et al. Ubiquitin-specific protease 4 antagonizes osteoblast differentiation through dishevelled [J]. J Bone Miner Res, 2016, 31(10):1888-1898.
[14] Lau AW, Pringle LM, Quick L, et al. TRE17/ubiquitin-specific protease 6 (USP6) oncogene translocated in aneurysmal bone cyst blocks osteoblastic maturation via an autocrine mechanism involving bone morphogenetic protein dysregulation[J]. J Biol Chem, 2010, 285(47):37111-37120.
[15] Aron R, Pellegrini P, Green EW, et al. Deubiquitinase Usp12 functions noncatalytically to induce autophagy and confer neuroprotection in models of Huntington's disease [J]. Nat Commun, 2020, 11(1):2374.
[16] Joo HY, Jones A, Yang C, et al. Regulation of Histone H2A and H2B Deubiquitination and Xenopus Development by USP12 and USP46 [J]. J Biol Chem, 2011, 286(9):7190-7201.
[17] Nayak TKS , Alamuru-Yellapragada NP , Parsa KVL . Deubiquitinase USP12 promotes LPS induced macrophage responses through inhibition of IκBα [J]. Biochem Biophys Res Commun, 2017, 483(1):69-74.