Biocompatibility of Ultrafine-grained Titanium Used for Implant after Sandblasting and Large-grits Acid-etching.

doi:10.11839/kqyxzh.2016.01.007

Abstract

Abstract: Objective: To study the effect of sandblasting and large-grits acid-etching (SLA) on the adhesion and proliferation of MC3T3-E1 cells on ultrafine-grained titanium (UFG Ti). Methods: Ti cylindrical specimen with 6mm in diameter and 3mm in height were incised from the bars of UFG Ti and Pure Ti. Experimental group (SLA-UFG Ti) was UFG Ti through SLA, while control group were UFG Ti through mechanical processing without any advanced processing (M-UFG Ti) and Pure Ti through SLA (SLA-Ti). The surface morphology and hydrophily of the surfaces were analyzed. The MC3T3-E1 cells were seeded on the surfaces of titanium specimens. Cell initial adhesion, cell density, and cell viability were observed. Results: Plenty of tiny sandpits with irregular shape were formed on the surface of SLA-UFG Ti group. Hydrophily of UFG Ti was improved after Equal-Channel Angular Pressing processing (ECAP). At the early stage, cell adhesion on the surface of SLA-UFG Ti was better than that of control group, while cell density was more intensive in the middle stage and the later stage. Cell viability was better at the middle stage, while no significant difference at the later stage. Conclusion: The surface property of SLA-UFG Ti was improved. SLA-UFG Ti could induce adhesion and proliferation of MC3T3-E1 cells. It might be a prospective substitute to pure Ti implant.

CLC Number:

R783.1

AN Bai-li, LI Zhi-rui, DIAO Xiao-ou, XIN Hai-tao, GUO Ya-zhou, WU Yu-lu.. Biocompatibility of Ultrafine-grained Titanium Used for Implant after Sandblasting and Large-grits Acid-etching.[J]. Journal of Oral Science Research, 2016, 32(1): 26-30.

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